Technical note: Use of internal transcribed spacer for ruminal yeast identification in dairy cows

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Standard

Technical note : Use of internal transcribed spacer for ruminal yeast identification in dairy cows. / Vargas-Bello-Pérez, E.; Cancino-Padilla, N.; Romero, J.

I: Animal, Bind 10, Nr. 12, 01.12.2016, s. 1949-1954.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Vargas-Bello-Pérez, E, Cancino-Padilla, N & Romero, J 2016, 'Technical note: Use of internal transcribed spacer for ruminal yeast identification in dairy cows', Animal, bind 10, nr. 12, s. 1949-1954. https://doi.org/10.1017/S1751731116000768

APA

Vargas-Bello-Pérez, E., Cancino-Padilla, N., & Romero, J. (2016). Technical note: Use of internal transcribed spacer for ruminal yeast identification in dairy cows. Animal, 10(12), 1949-1954. https://doi.org/10.1017/S1751731116000768

Vancouver

Vargas-Bello-Pérez E, Cancino-Padilla N, Romero J. Technical note: Use of internal transcribed spacer for ruminal yeast identification in dairy cows. Animal. 2016 dec. 1;10(12):1949-1954. https://doi.org/10.1017/S1751731116000768

Author

Vargas-Bello-Pérez, E. ; Cancino-Padilla, N. ; Romero, J. / Technical note : Use of internal transcribed spacer for ruminal yeast identification in dairy cows. I: Animal. 2016 ; Bind 10, Nr. 12. s. 1949-1954.

Bibtex

@article{00d949eb5977440ea790e17c869e113a,
title = "Technical note: Use of internal transcribed spacer for ruminal yeast identification in dairy cows",
abstract = "Molecular techniques are important tools for microbiological studies in different habitats, and the internal transcribed spacer (ITS) has been proved to be useful for analyzing fungal diversity. The aim of this study was to use the ITS region to generate ruminal yeast profile and to identify ruminal yeast. DNA from ruminal digesta was extracted to amplify the ribosomal ITS region. The profile from the PCR products was visualized and the excised bands from the profile were identified as the genera Millerozyma, Pichia, Rhizomucor and Hyphopichia. Overall, the ITS resulted to be a simple, fast and sensitive approach that allowed profiling and identification of ruminal yeast that have not been previously described (Millerozyma and Hyphopichia) in the rumen microbial community.",
keywords = "dairy cow, internal transcribed spacer, Keywords rumen, yeast identification",
author = "E. Vargas-Bello-P{\'e}rez and N. Cancino-Padilla and J. Romero",
year = "2016",
month = dec,
day = "1",
doi = "10.1017/S1751731116000768",
language = "English",
volume = "10",
pages = "1949--1954",
journal = "Animal",
issn = "1751-7311",
publisher = "Cambridge University Press",
number = "12",

}

RIS

TY - JOUR

T1 - Technical note

T2 - Use of internal transcribed spacer for ruminal yeast identification in dairy cows

AU - Vargas-Bello-Pérez, E.

AU - Cancino-Padilla, N.

AU - Romero, J.

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Molecular techniques are important tools for microbiological studies in different habitats, and the internal transcribed spacer (ITS) has been proved to be useful for analyzing fungal diversity. The aim of this study was to use the ITS region to generate ruminal yeast profile and to identify ruminal yeast. DNA from ruminal digesta was extracted to amplify the ribosomal ITS region. The profile from the PCR products was visualized and the excised bands from the profile were identified as the genera Millerozyma, Pichia, Rhizomucor and Hyphopichia. Overall, the ITS resulted to be a simple, fast and sensitive approach that allowed profiling and identification of ruminal yeast that have not been previously described (Millerozyma and Hyphopichia) in the rumen microbial community.

AB - Molecular techniques are important tools for microbiological studies in different habitats, and the internal transcribed spacer (ITS) has been proved to be useful for analyzing fungal diversity. The aim of this study was to use the ITS region to generate ruminal yeast profile and to identify ruminal yeast. DNA from ruminal digesta was extracted to amplify the ribosomal ITS region. The profile from the PCR products was visualized and the excised bands from the profile were identified as the genera Millerozyma, Pichia, Rhizomucor and Hyphopichia. Overall, the ITS resulted to be a simple, fast and sensitive approach that allowed profiling and identification of ruminal yeast that have not been previously described (Millerozyma and Hyphopichia) in the rumen microbial community.

KW - dairy cow

KW - internal transcribed spacer

KW - Keywords rumen

KW - yeast identification

U2 - 10.1017/S1751731116000768

DO - 10.1017/S1751731116000768

M3 - Journal article

C2 - 27133003

AN - SCOPUS:84964766349

VL - 10

SP - 1949

EP - 1954

JO - Animal

JF - Animal

SN - 1751-7311

IS - 12

ER -

ID: 194028716