Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray
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Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray. / Skottrup, Peter Durand; Hansen, Mikkel Fougt; Moresco, Jacob Lange; Deryabina, Maria; Svendsen, Winnie Edith; Jakobsen, Mogens Havsteen; Dufva, Martin.
I: Acta Biomaterialia, Bind 6, Nr. 10, 2010, s. 3936-3946.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray
AU - Skottrup, Peter Durand
AU - Hansen, Mikkel Fougt
AU - Moresco, Jacob Lange
AU - Deryabina, Maria
AU - Svendsen, Winnie Edith
AU - Jakobsen, Mogens Havsteen
AU - Dufva, Martin
PY - 2010
Y1 - 2010
N2 - Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO2 with or without surface modification SiO2 surface cleaning, immobilized substrate concentration and surface blocking conditions were optimized Polyethylene glycol-based surfaces with different end groups on the anchor molecule, 2,4,6-trichloro-1,3,5-triazine (TsT), were synthesized and compared with the standard (3-aminopropyl)triethoxysilane (APTS)/glutaraldehyde chemistry APTS/glutaraldehyde, directly linked TsT and bare H2O2-activated SiO2 performed better than polyethylene glycol-modified surfaces Two beads, Masterbeads and M-280 beads, were found to give superior results compared with other bead types. Antibody/ antigen interactions, Illustrated by C-reactive protein, were best performed with Masterbeads The results provide important information concerning the surface binding properties of streptavidin-functionalized beads and the immunomicroarray can be used when optimizing the performance of bead-based biosensors.
AB - Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO2 with or without surface modification SiO2 surface cleaning, immobilized substrate concentration and surface blocking conditions were optimized Polyethylene glycol-based surfaces with different end groups on the anchor molecule, 2,4,6-trichloro-1,3,5-triazine (TsT), were synthesized and compared with the standard (3-aminopropyl)triethoxysilane (APTS)/glutaraldehyde chemistry APTS/glutaraldehyde, directly linked TsT and bare H2O2-activated SiO2 performed better than polyethylene glycol-modified surfaces Two beads, Masterbeads and M-280 beads, were found to give superior results compared with other bead types. Antibody/ antigen interactions, Illustrated by C-reactive protein, were best performed with Masterbeads The results provide important information concerning the surface binding properties of streptavidin-functionalized beads and the immunomicroarray can be used when optimizing the performance of bead-based biosensors.
KW - Former Faculty of Pharmaceutical Sciences
M3 - Journal article
VL - 6
SP - 3936
EP - 3946
JO - Acta Biomaterialia
JF - Acta Biomaterialia
SN - 1742-7061
IS - 10
ER -
ID: 33238365