Studies on the oxidizing system in Holt's medium for histochemical demonstration of esterase activity
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Studies on the oxidizing system in Holt's medium for histochemical demonstration of esterase activity. / Kirkeby, S; Blecher, S R.
I: Acta Histochemica, Bind 62, Nr. 1, 1978, s. 44-56.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Studies on the oxidizing system in Holt's medium for histochemical demonstration of esterase activity
AU - Kirkeby, S
AU - Blecher, S R
N1 - Keywords: Animals; Culture Media; Epididymis; Esterases; Ferricyanides; Ferrocyanides; Guinea Pigs; Histocytochemistry; Male; Mice; Oxidation-Reduction; Sulfhydryl Compounds; Thyroid Gland
PY - 1978
Y1 - 1978
N2 - Esterase activity in guinea-pig thyroid and mouse epididymis epithelial cells has been studied using 5-bromoindoxyl acetate as substrate. The pattern of esterase activity in the thyroid of the guinea-pig is constant, irrespective of whether ferri-ferrocyanide (FFC) or certain copper compounds are used as oxidizing agents in the incubation medium. The intensity of the coloured reaction product is increased when cobalt or manganese are added to the incubation medium. Activity is depressed by high concentrations of FFC when resent in incubation medium or preincubational buffer only. Epididymis cells contain an esterase activity which is not inhibited by conventional SH blocking agents, nor by high concentrations of FFC. From these results it appears that the mode of action of FFC in Holt's medium is as follows. At low concentrations FFC appears to act primarily as a catalytic agent in oxidation of indoxyl to indigoid. At high concentration FFC acts as an inhibitor of guinea-pig thyroid esterase, by oxidation of SH groups in the active centre. The esterase of mouse epididymis cell type EH 1 is not subject to this inhibition by FFC, presumably because it does not contain accessible SH groups in the active centre.
AB - Esterase activity in guinea-pig thyroid and mouse epididymis epithelial cells has been studied using 5-bromoindoxyl acetate as substrate. The pattern of esterase activity in the thyroid of the guinea-pig is constant, irrespective of whether ferri-ferrocyanide (FFC) or certain copper compounds are used as oxidizing agents in the incubation medium. The intensity of the coloured reaction product is increased when cobalt or manganese are added to the incubation medium. Activity is depressed by high concentrations of FFC when resent in incubation medium or preincubational buffer only. Epididymis cells contain an esterase activity which is not inhibited by conventional SH blocking agents, nor by high concentrations of FFC. From these results it appears that the mode of action of FFC in Holt's medium is as follows. At low concentrations FFC appears to act primarily as a catalytic agent in oxidation of indoxyl to indigoid. At high concentration FFC acts as an inhibitor of guinea-pig thyroid esterase, by oxidation of SH groups in the active centre. The esterase of mouse epididymis cell type EH 1 is not subject to this inhibition by FFC, presumably because it does not contain accessible SH groups in the active centre.
M3 - Journal article
C2 - 99964
VL - 62
SP - 44
EP - 56
JO - Acta Histochemica
JF - Acta Histochemica
SN - 0065-1281
IS - 1
ER -
ID: 10252971