Skin barrier damage after exposure to paraphenylenediamine
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Skin barrier damage after exposure to paraphenylenediamine. / Meisser, Sanne S; Altunbulakli, Can; Bandier, Josefine; Opstrup, Morten S; Castro-Giner, Francesc; Akdis, Mübeccel; Bonefeld, Charlotte M; Johansen, Jeanne D; Akdis, Cezmi A.
I: The Journal of allergy and clinical immunology, Bind 145, Nr. 2, 2020, s. 619-631.e2.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Skin barrier damage after exposure to paraphenylenediamine
AU - Meisser, Sanne S
AU - Altunbulakli, Can
AU - Bandier, Josefine
AU - Opstrup, Morten S
AU - Castro-Giner, Francesc
AU - Akdis, Mübeccel
AU - Bonefeld, Charlotte M
AU - Johansen, Jeanne D
AU - Akdis, Cezmi A
N1 - Copyright © 2019 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.
PY - 2020
Y1 - 2020
N2 - BACKGROUND: p-Phenylenediamine (PPD) is a strong contact allergen used in hair dye that is known to cause allergic contact dermatitis (ACD). Both private and occupational exposure to PPD is frequent, but the effect of PPD exposure in nonallergic occupationally exposed subjects is unknown.OBJECTIVE: We sought to investigate the effects of PPD exposure on the skin of occupationally exposed subjects with and without clinical symptoms.METHODS: Skin biopsy specimens were collected from 4 patients with mild and 5 patients with severe PPD-related ACD and 7 hairdressers without contact dermatitis on day 4 after patch testing with 1% PPD in petrolatum. RNA sequencing and transcriptomics analyses were performed and confirmed by using quantitative RT-PCR. Protein expression was analyzed in skin from 4 hairdressers and 1 patient with ACD by using immunofluorescence staining. Reconstructed human epidermis was used to test the effects of PPD in vitro.RESULTS: RNA sequencing demonstrated downregulation of tight junction and stratum corneum proteins in the skin of patients with severe ACD after PPD exposure. Claudin-1 (CLDN-1), CLDN8, CLDN11, CXADR-like membrane protein (CLMP), occludin (OCLN), membrane-associated guanylate kinase inverted 1 (MAGI1), and MAGI2 mRNA expression was downregulated in patients with severe ACD. CLDN1 and CLMP expression were downregulated in nonresponding hairdressers and patients with mild ACD. Filaggrin 1 (FLG1), FLG2, and loricrin (LOR) expression were downregulated in patients with ACD. Confocal microscopic images showed downregulation of CLDN-1, FLG-1, and FLG-2 expression. In contrast, 3-dimensional skin cultures showed upregulation of FLG-1 in response to PPD but downregulation of FLG-2.CONCLUSION: PPD-exposed skin is associated with extensive transcriptomic changes, including downregulation of tight junction and stratum corneum proteins, even in the absence of clinical symptoms.
AB - BACKGROUND: p-Phenylenediamine (PPD) is a strong contact allergen used in hair dye that is known to cause allergic contact dermatitis (ACD). Both private and occupational exposure to PPD is frequent, but the effect of PPD exposure in nonallergic occupationally exposed subjects is unknown.OBJECTIVE: We sought to investigate the effects of PPD exposure on the skin of occupationally exposed subjects with and without clinical symptoms.METHODS: Skin biopsy specimens were collected from 4 patients with mild and 5 patients with severe PPD-related ACD and 7 hairdressers without contact dermatitis on day 4 after patch testing with 1% PPD in petrolatum. RNA sequencing and transcriptomics analyses were performed and confirmed by using quantitative RT-PCR. Protein expression was analyzed in skin from 4 hairdressers and 1 patient with ACD by using immunofluorescence staining. Reconstructed human epidermis was used to test the effects of PPD in vitro.RESULTS: RNA sequencing demonstrated downregulation of tight junction and stratum corneum proteins in the skin of patients with severe ACD after PPD exposure. Claudin-1 (CLDN-1), CLDN8, CLDN11, CXADR-like membrane protein (CLMP), occludin (OCLN), membrane-associated guanylate kinase inverted 1 (MAGI1), and MAGI2 mRNA expression was downregulated in patients with severe ACD. CLDN1 and CLMP expression were downregulated in nonresponding hairdressers and patients with mild ACD. Filaggrin 1 (FLG1), FLG2, and loricrin (LOR) expression were downregulated in patients with ACD. Confocal microscopic images showed downregulation of CLDN-1, FLG-1, and FLG-2 expression. In contrast, 3-dimensional skin cultures showed upregulation of FLG-1 in response to PPD but downregulation of FLG-2.CONCLUSION: PPD-exposed skin is associated with extensive transcriptomic changes, including downregulation of tight junction and stratum corneum proteins, even in the absence of clinical symptoms.
KW - Adult
KW - Dermatitis, Allergic Contact/etiology
KW - Female
KW - Hair Dyes/adverse effects
KW - Humans
KW - Occupational Exposure/adverse effects
KW - Phenylenediamines/adverse effects
KW - Skin/drug effects
KW - Tight Junction Proteins/drug effects
U2 - 10.1016/j.jaci.2019.11.023
DO - 10.1016/j.jaci.2019.11.023
M3 - Journal article
C2 - 31783056
VL - 145
SP - 619-631.e2
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
SN - 0091-6749
IS - 2
ER -
ID: 257659298