Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems
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Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems. / Ahmerkamp, Soeren; Jalaluddin, Farooq Moin; Cui, Yuan; Brumley, Douglas R.; Pacherres, Cesar O.; Berg, Jasmine S.; Stocker, Roman; Kuypers, Marcel M. M.; Koren, Klaus; Behrendt, Lars.
I: Cell Reports Methods, Bind 2, Nr. 5, 100216, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems
AU - Ahmerkamp, Soeren
AU - Jalaluddin, Farooq Moin
AU - Cui, Yuan
AU - Brumley, Douglas R.
AU - Pacherres, Cesar O.
AU - Berg, Jasmine S.
AU - Stocker, Roman
AU - Kuypers, Marcel M. M.
AU - Koren, Klaus
AU - Behrendt, Lars
PY - 2022
Y1 - 2022
N2 - From individual cells to whole organisms, O-2 transport unfolds across micrometer- tomillimeter-length scales and can change within milliseconds in response to fluid flows and organismal behavior. The spatiotemporal complexity of these processes makes the accurate assessment of O-2 dynamics via currently availablemethods difficult or unreliable. Here, we present "sensPIV,'' a method to simultaneously measure O-2 concentrations and flow fields. By tracking O-2-sensitive microparticles in flow using imaging technologies that allow for instantaneous referencing, wemeasuredO(2) transportwithin (1) microfluidic devices, (2) sinkingmodel aggregates, and (3) complex colony-forming corals. Through the use of sensPIV, we find that corals use ciliarymovement to link zones of photosynthetic O-2 production to zones of O-2 consumption. SensPIV can potentially be extendable to study flow-organism interactions across many life-science and engineering applications.
AB - From individual cells to whole organisms, O-2 transport unfolds across micrometer- tomillimeter-length scales and can change within milliseconds in response to fluid flows and organismal behavior. The spatiotemporal complexity of these processes makes the accurate assessment of O-2 dynamics via currently availablemethods difficult or unreliable. Here, we present "sensPIV,'' a method to simultaneously measure O-2 concentrations and flow fields. By tracking O-2-sensitive microparticles in flow using imaging technologies that allow for instantaneous referencing, wemeasuredO(2) transportwithin (1) microfluidic devices, (2) sinkingmodel aggregates, and (3) complex colony-forming corals. Through the use of sensPIV, we find that corals use ciliarymovement to link zones of photosynthetic O-2 production to zones of O-2 consumption. SensPIV can potentially be extendable to study flow-organism interactions across many life-science and engineering applications.
KW - PARTICLE IMAGE VELOCIMETRY
KW - O-2 DYNAMICS
KW - FLUID
KW - RHIZOSPHERE
KW - VERSATILE
KW - SEDIMENTS
KW - FLUXES
KW - ROOTS
KW - CELLS
KW - LIVE
U2 - 10.1016/j.crmeth.2022.100216
DO - 10.1016/j.crmeth.2022.100216
M3 - Journal article
C2 - 35637907
VL - 2
JO - Cell Reports Methods
JF - Cell Reports Methods
SN - 2667-2375
IS - 5
M1 - 100216
ER -
ID: 335675957