Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor
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Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor. / Donia, Marco; Larsen, Signe Møllebæk; Met, Ozcan; Svane, Inge Marie.
I: Cytotherapy, Bind 16, Nr. 8, 08.2014, s. 1117–1120.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor
AU - Donia, Marco
AU - Larsen, Signe Møllebæk
AU - Met, Ozcan
AU - Svane, Inge Marie
N1 - Copyright © 2014 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.
PY - 2014/8
Y1 - 2014/8
N2 - BACKGROUND AIMS: The high level of complexity of current Good Manufacturing Practice-compliant methods of manufacturing hampers rapid and broad application of treatment with tumor-infiltrating lymphocytes (TILs).METHODS: To ensure higher applicability of TIL production to laboratory routine, a practical and simple protocol of TIL manufacturing with the use of a closed-system bioreactor was developed and implemented at our institution.RESULTS: This protocol enabled significant work load reduction during the most labor-intense step of TIL expansion, and allowed generation of high-quality TIL products, which mediated clinical regression in patients with metastatic melanoma.CONCLUSIONS: Implementation of simplified methods of TIL expansion will speed up dissemination of TIL methods worldwide and will increase patient access to this highly effective treatment.
AB - BACKGROUND AIMS: The high level of complexity of current Good Manufacturing Practice-compliant methods of manufacturing hampers rapid and broad application of treatment with tumor-infiltrating lymphocytes (TILs).METHODS: To ensure higher applicability of TIL production to laboratory routine, a practical and simple protocol of TIL manufacturing with the use of a closed-system bioreactor was developed and implemented at our institution.RESULTS: This protocol enabled significant work load reduction during the most labor-intense step of TIL expansion, and allowed generation of high-quality TIL products, which mediated clinical regression in patients with metastatic melanoma.CONCLUSIONS: Implementation of simplified methods of TIL expansion will speed up dissemination of TIL methods worldwide and will increase patient access to this highly effective treatment.
KW - Bioreactors
KW - CD8-Positive T-Lymphocytes
KW - Cell Proliferation
KW - Cell- and Tissue-Based Therapy
KW - Humans
KW - Immunotherapy, Adoptive
KW - Lymphocytes, Tumor-Infiltrating
KW - Melanoma
KW - Neoplasm Metastasis
U2 - 10.1016/j.jcyt.2014.02.004
DO - 10.1016/j.jcyt.2014.02.004
M3 - Journal article
C2 - 24831841
VL - 16
SP - 1117
EP - 1120
JO - Cytotherapy
JF - Cytotherapy
SN - 1465-3249
IS - 8
ER -
ID: 138382344