Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor

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Standard

Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor. / Donia, Marco; Larsen, Signe Møllebæk; Met, Ozcan; Svane, Inge Marie.

I: Cytotherapy, Bind 16, Nr. 8, 08.2014, s. 1117–1120.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Donia, M, Larsen, SM, Met, O & Svane, IM 2014, 'Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor', Cytotherapy, bind 16, nr. 8, s. 1117–1120. https://doi.org/10.1016/j.jcyt.2014.02.004

APA

Donia, M., Larsen, S. M., Met, O., & Svane, I. M. (2014). Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor. Cytotherapy, 16(8), 1117–1120. https://doi.org/10.1016/j.jcyt.2014.02.004

Vancouver

Donia M, Larsen SM, Met O, Svane IM. Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor. Cytotherapy. 2014 aug.;16(8):1117–1120. https://doi.org/10.1016/j.jcyt.2014.02.004

Author

Donia, Marco ; Larsen, Signe Møllebæk ; Met, Ozcan ; Svane, Inge Marie. / Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor. I: Cytotherapy. 2014 ; Bind 16, Nr. 8. s. 1117–1120.

Bibtex

@article{9bb58f73f99e489da8e22e2497f6943c,
title = "Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor",
abstract = "BACKGROUND AIMS: The high level of complexity of current Good Manufacturing Practice-compliant methods of manufacturing hampers rapid and broad application of treatment with tumor-infiltrating lymphocytes (TILs).METHODS: To ensure higher applicability of TIL production to laboratory routine, a practical and simple protocol of TIL manufacturing with the use of a closed-system bioreactor was developed and implemented at our institution.RESULTS: This protocol enabled significant work load reduction during the most labor-intense step of TIL expansion, and allowed generation of high-quality TIL products, which mediated clinical regression in patients with metastatic melanoma.CONCLUSIONS: Implementation of simplified methods of TIL expansion will speed up dissemination of TIL methods worldwide and will increase patient access to this highly effective treatment.",
keywords = "Bioreactors, CD8-Positive T-Lymphocytes, Cell Proliferation, Cell- and Tissue-Based Therapy, Humans, Immunotherapy, Adoptive, Lymphocytes, Tumor-Infiltrating, Melanoma, Neoplasm Metastasis",
author = "Marco Donia and Larsen, {Signe M{\o}lleb{\ae}k} and Ozcan Met and Svane, {Inge Marie}",
note = "Copyright {\textcopyright} 2014 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.",
year = "2014",
month = aug,
doi = "10.1016/j.jcyt.2014.02.004",
language = "English",
volume = "16",
pages = "1117–1120",
journal = "Cytotherapy",
issn = "1465-3249",
publisher = "Elsevier",
number = "8",

}

RIS

TY - JOUR

T1 - Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor

AU - Donia, Marco

AU - Larsen, Signe Møllebæk

AU - Met, Ozcan

AU - Svane, Inge Marie

N1 - Copyright © 2014 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

PY - 2014/8

Y1 - 2014/8

N2 - BACKGROUND AIMS: The high level of complexity of current Good Manufacturing Practice-compliant methods of manufacturing hampers rapid and broad application of treatment with tumor-infiltrating lymphocytes (TILs).METHODS: To ensure higher applicability of TIL production to laboratory routine, a practical and simple protocol of TIL manufacturing with the use of a closed-system bioreactor was developed and implemented at our institution.RESULTS: This protocol enabled significant work load reduction during the most labor-intense step of TIL expansion, and allowed generation of high-quality TIL products, which mediated clinical regression in patients with metastatic melanoma.CONCLUSIONS: Implementation of simplified methods of TIL expansion will speed up dissemination of TIL methods worldwide and will increase patient access to this highly effective treatment.

AB - BACKGROUND AIMS: The high level of complexity of current Good Manufacturing Practice-compliant methods of manufacturing hampers rapid and broad application of treatment with tumor-infiltrating lymphocytes (TILs).METHODS: To ensure higher applicability of TIL production to laboratory routine, a practical and simple protocol of TIL manufacturing with the use of a closed-system bioreactor was developed and implemented at our institution.RESULTS: This protocol enabled significant work load reduction during the most labor-intense step of TIL expansion, and allowed generation of high-quality TIL products, which mediated clinical regression in patients with metastatic melanoma.CONCLUSIONS: Implementation of simplified methods of TIL expansion will speed up dissemination of TIL methods worldwide and will increase patient access to this highly effective treatment.

KW - Bioreactors

KW - CD8-Positive T-Lymphocytes

KW - Cell Proliferation

KW - Cell- and Tissue-Based Therapy

KW - Humans

KW - Immunotherapy, Adoptive

KW - Lymphocytes, Tumor-Infiltrating

KW - Melanoma

KW - Neoplasm Metastasis

U2 - 10.1016/j.jcyt.2014.02.004

DO - 10.1016/j.jcyt.2014.02.004

M3 - Journal article

C2 - 24831841

VL - 16

SP - 1117

EP - 1120

JO - Cytotherapy

JF - Cytotherapy

SN - 1465-3249

IS - 8

ER -

ID: 138382344