Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115

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Standard

Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115. / Shorter, James; Beard, Matthew B; Seemann, Joachim; Dirac-Svejstrup, A Barbara; Warren, Graham.

I: The Journal of Cell Biology, Bind 157, Nr. 1, 01.04.2002, s. 45-62.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Shorter, J, Beard, MB, Seemann, J, Dirac-Svejstrup, AB & Warren, G 2002, 'Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115', The Journal of Cell Biology, bind 157, nr. 1, s. 45-62. https://doi.org/10.1083/jcb.200112127

APA

Shorter, J., Beard, M. B., Seemann, J., Dirac-Svejstrup, A. B., & Warren, G. (2002). Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115. The Journal of Cell Biology, 157(1), 45-62. https://doi.org/10.1083/jcb.200112127

Vancouver

Shorter J, Beard MB, Seemann J, Dirac-Svejstrup AB, Warren G. Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115. The Journal of Cell Biology. 2002 apr. 1;157(1):45-62. https://doi.org/10.1083/jcb.200112127

Author

Shorter, James ; Beard, Matthew B ; Seemann, Joachim ; Dirac-Svejstrup, A Barbara ; Warren, Graham. / Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115. I: The Journal of Cell Biology. 2002 ; Bind 157, Nr. 1. s. 45-62.

Bibtex

@article{31db8d65b15c4888a314fc8f1246001c,
title = "Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115",
abstract = "p115 tethers coat protein (COP)I vesicles to Golgi membranes. The acidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi membranes. We now show that a SNARE motif-related domain within p115 stimulates the specific assembly of endogenous Golgi SNAREpins containing the t-SNARE, syntaxin 5. p115 catalyzes the construction of a cognate GOS-28-syntaxin-5 (v-/t-SNARE) complex by first linking the SNAREs to promote their direct interaction. These events are essential for NSF-catalyzed reassembly of postmitotic Golgi vesicles and tubules into mature cisternae. Staging experiments reveal that the linking of Golgins precedes SNAREpin assembly. Thus, p115 coordinates sequential tethering and docking of COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs).",
keywords = "Animals, Autoantigens, COP-Coated Vesicles/metabolism, Carrier Proteins/metabolism, Detergents, Golgi Apparatus/metabolism, Golgi Matrix Proteins, In Vitro Techniques, Membrane Fusion/physiology, Membrane Proteins/metabolism, Qa-SNARE Proteins, Qb-SNARE Proteins, Qc-SNARE Proteins, R-SNARE Proteins, Rats, Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins, Vesicular Transport Proteins",
author = "James Shorter and Beard, {Matthew B} and Joachim Seemann and Dirac-Svejstrup, {A Barbara} and Graham Warren",
year = "2002",
month = apr,
day = "1",
doi = "10.1083/jcb.200112127",
language = "English",
volume = "157",
pages = "45--62",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115

AU - Shorter, James

AU - Beard, Matthew B

AU - Seemann, Joachim

AU - Dirac-Svejstrup, A Barbara

AU - Warren, Graham

PY - 2002/4/1

Y1 - 2002/4/1

N2 - p115 tethers coat protein (COP)I vesicles to Golgi membranes. The acidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi membranes. We now show that a SNARE motif-related domain within p115 stimulates the specific assembly of endogenous Golgi SNAREpins containing the t-SNARE, syntaxin 5. p115 catalyzes the construction of a cognate GOS-28-syntaxin-5 (v-/t-SNARE) complex by first linking the SNAREs to promote their direct interaction. These events are essential for NSF-catalyzed reassembly of postmitotic Golgi vesicles and tubules into mature cisternae. Staging experiments reveal that the linking of Golgins precedes SNAREpin assembly. Thus, p115 coordinates sequential tethering and docking of COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs).

AB - p115 tethers coat protein (COP)I vesicles to Golgi membranes. The acidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi membranes. We now show that a SNARE motif-related domain within p115 stimulates the specific assembly of endogenous Golgi SNAREpins containing the t-SNARE, syntaxin 5. p115 catalyzes the construction of a cognate GOS-28-syntaxin-5 (v-/t-SNARE) complex by first linking the SNAREs to promote their direct interaction. These events are essential for NSF-catalyzed reassembly of postmitotic Golgi vesicles and tubules into mature cisternae. Staging experiments reveal that the linking of Golgins precedes SNAREpin assembly. Thus, p115 coordinates sequential tethering and docking of COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs).

KW - Animals

KW - Autoantigens

KW - COP-Coated Vesicles/metabolism

KW - Carrier Proteins/metabolism

KW - Detergents

KW - Golgi Apparatus/metabolism

KW - Golgi Matrix Proteins

KW - In Vitro Techniques

KW - Membrane Fusion/physiology

KW - Membrane Proteins/metabolism

KW - Qa-SNARE Proteins

KW - Qb-SNARE Proteins

KW - Qc-SNARE Proteins

KW - R-SNARE Proteins

KW - Rats

KW - Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins

KW - Vesicular Transport Proteins

U2 - 10.1083/jcb.200112127

DO - 10.1083/jcb.200112127

M3 - Journal article

C2 - 11927603

VL - 157

SP - 45

EP - 62

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 1

ER -

ID: 333770188