Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115
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Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115. / Shorter, James; Beard, Matthew B; Seemann, Joachim; Dirac-Svejstrup, A Barbara; Warren, Graham.
I: The Journal of Cell Biology, Bind 157, Nr. 1, 01.04.2002, s. 45-62.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115
AU - Shorter, James
AU - Beard, Matthew B
AU - Seemann, Joachim
AU - Dirac-Svejstrup, A Barbara
AU - Warren, Graham
PY - 2002/4/1
Y1 - 2002/4/1
N2 - p115 tethers coat protein (COP)I vesicles to Golgi membranes. The acidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi membranes. We now show that a SNARE motif-related domain within p115 stimulates the specific assembly of endogenous Golgi SNAREpins containing the t-SNARE, syntaxin 5. p115 catalyzes the construction of a cognate GOS-28-syntaxin-5 (v-/t-SNARE) complex by first linking the SNAREs to promote their direct interaction. These events are essential for NSF-catalyzed reassembly of postmitotic Golgi vesicles and tubules into mature cisternae. Staging experiments reveal that the linking of Golgins precedes SNAREpin assembly. Thus, p115 coordinates sequential tethering and docking of COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs).
AB - p115 tethers coat protein (COP)I vesicles to Golgi membranes. The acidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi membranes. We now show that a SNARE motif-related domain within p115 stimulates the specific assembly of endogenous Golgi SNAREpins containing the t-SNARE, syntaxin 5. p115 catalyzes the construction of a cognate GOS-28-syntaxin-5 (v-/t-SNARE) complex by first linking the SNAREs to promote their direct interaction. These events are essential for NSF-catalyzed reassembly of postmitotic Golgi vesicles and tubules into mature cisternae. Staging experiments reveal that the linking of Golgins precedes SNAREpin assembly. Thus, p115 coordinates sequential tethering and docking of COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs).
KW - Animals
KW - Autoantigens
KW - COP-Coated Vesicles/metabolism
KW - Carrier Proteins/metabolism
KW - Detergents
KW - Golgi Apparatus/metabolism
KW - Golgi Matrix Proteins
KW - In Vitro Techniques
KW - Membrane Fusion/physiology
KW - Membrane Proteins/metabolism
KW - Qa-SNARE Proteins
KW - Qb-SNARE Proteins
KW - Qc-SNARE Proteins
KW - R-SNARE Proteins
KW - Rats
KW - Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
KW - Vesicular Transport Proteins
U2 - 10.1083/jcb.200112127
DO - 10.1083/jcb.200112127
M3 - Journal article
C2 - 11927603
VL - 157
SP - 45
EP - 62
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 1
ER -
ID: 333770188