SCAF4 and SCAF8, mRNA Anti-Terminator Proteins

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SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. / Gregersen, Lea H.; Mitter, Richard; Ugalde, Alejandro P; Nojima, Takayuki; Proudfoot, Nicholas J; Agami, Reuven; Stewart, Aengus; Svejstrup, Jesper Q.

I: Cell, Bind 177, Nr. 7, 2019, s. 1797-1813.e18.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Gregersen, LH, Mitter, R, Ugalde, AP, Nojima, T, Proudfoot, NJ, Agami, R, Stewart, A & Svejstrup, JQ 2019, 'SCAF4 and SCAF8, mRNA Anti-Terminator Proteins', Cell, bind 177, nr. 7, s. 1797-1813.e18. https://doi.org/10.1016/j.cell.2019.04.038

APA

Gregersen, L. H., Mitter, R., Ugalde, A. P., Nojima, T., Proudfoot, N. J., Agami, R., Stewart, A., & Svejstrup, J. Q. (2019). SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. Cell, 177(7), 1797-1813.e18. https://doi.org/10.1016/j.cell.2019.04.038

Vancouver

Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R o.a. SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. Cell. 2019;177(7):1797-1813.e18. https://doi.org/10.1016/j.cell.2019.04.038

Author

Gregersen, Lea H. ; Mitter, Richard ; Ugalde, Alejandro P ; Nojima, Takayuki ; Proudfoot, Nicholas J ; Agami, Reuven ; Stewart, Aengus ; Svejstrup, Jesper Q. / SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. I: Cell. 2019 ; Bind 177, Nr. 7. s. 1797-1813.e18.

Bibtex

@article{8fa6fd7ce25c420f82aac9f02637b228,

title = "SCAF4 and SCAF8, mRNA Anti-Terminator Proteins",

abstract = "Accurate regulation of mRNA termination is required for correct gene expression. Here, we describe a role for SCAF4 and SCAF8 as anti-terminators, suppressing the use of early, alternative polyadenylation (polyA) sites. The SCAF4/8 proteins bind the hyper-phosphorylated RNAPII C-terminal repeat domain (CTD) phosphorylated on both Ser2 and Ser5 and are detected at early, alternative polyA sites. Concomitant knockout of human SCAF4 and SCAF8 results in altered polyA selection and subsequent early termination, leading to expression of truncated mRNAs and proteins lacking functional domains and is cell lethal. While SCAF4 and SCAF8 work redundantly to suppress early mRNA termination, they also have independent, non-essential functions. SCAF8 is an RNAPII elongation factor, whereas SCAF4 is required for correct termination at canonical, distal transcription termination sites in the presence of SCAF8. Together, SCAF4 and SCAF8 coordinate the transition between elongation and termination, ensuring correct polyA site selection and RNAPII transcriptional termination in human cells.",

keywords = "HEK293 Cells, Humans, Poly A/genetics, Protein Domains, RNA Polymerase II/genetics, RNA, Messenger/biosynthesis, RNA-Binding Proteins/genetics, Serine-Arginine Splicing Factors/genetics, Transcription Elongation, Genetic, Transcription Termination, Genetic",

author = "Gregersen, {Lea H.} and Richard Mitter and Ugalde, {Alejandro P} and Takayuki Nojima and Proudfoot, {Nicholas J} and Reuven Agami and Aengus Stewart and Svejstrup, {Jesper Q}",

year = "2019",

doi = "10.1016/j.cell.2019.04.038",

language = "English",

volume = "177",

pages = "1797--1813.e18",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "7",

}

RIS

TY - JOUR

T1 - SCAF4 and SCAF8, mRNA Anti-Terminator Proteins

AU - Gregersen, Lea H.

AU - Mitter, Richard

AU - Ugalde, Alejandro P

AU - Nojima, Takayuki

AU - Proudfoot, Nicholas J

AU - Agami, Reuven

AU - Stewart, Aengus

AU - Svejstrup, Jesper Q

PY - 2019

Y1 - 2019

N2 - Accurate regulation of mRNA termination is required for correct gene expression. Here, we describe a role for SCAF4 and SCAF8 as anti-terminators, suppressing the use of early, alternative polyadenylation (polyA) sites. The SCAF4/8 proteins bind the hyper-phosphorylated RNAPII C-terminal repeat domain (CTD) phosphorylated on both Ser2 and Ser5 and are detected at early, alternative polyA sites. Concomitant knockout of human SCAF4 and SCAF8 results in altered polyA selection and subsequent early termination, leading to expression of truncated mRNAs and proteins lacking functional domains and is cell lethal. While SCAF4 and SCAF8 work redundantly to suppress early mRNA termination, they also have independent, non-essential functions. SCAF8 is an RNAPII elongation factor, whereas SCAF4 is required for correct termination at canonical, distal transcription termination sites in the presence of SCAF8. Together, SCAF4 and SCAF8 coordinate the transition between elongation and termination, ensuring correct polyA site selection and RNAPII transcriptional termination in human cells.

AB - Accurate regulation of mRNA termination is required for correct gene expression. Here, we describe a role for SCAF4 and SCAF8 as anti-terminators, suppressing the use of early, alternative polyadenylation (polyA) sites. The SCAF4/8 proteins bind the hyper-phosphorylated RNAPII C-terminal repeat domain (CTD) phosphorylated on both Ser2 and Ser5 and are detected at early, alternative polyA sites. Concomitant knockout of human SCAF4 and SCAF8 results in altered polyA selection and subsequent early termination, leading to expression of truncated mRNAs and proteins lacking functional domains and is cell lethal. While SCAF4 and SCAF8 work redundantly to suppress early mRNA termination, they also have independent, non-essential functions. SCAF8 is an RNAPII elongation factor, whereas SCAF4 is required for correct termination at canonical, distal transcription termination sites in the presence of SCAF8. Together, SCAF4 and SCAF8 coordinate the transition between elongation and termination, ensuring correct polyA site selection and RNAPII transcriptional termination in human cells.

KW - HEK293 Cells

KW - Humans

KW - Poly A/genetics

KW - Protein Domains

KW - RNA Polymerase II/genetics

KW - RNA, Messenger/biosynthesis

KW - RNA-Binding Proteins/genetics

KW - Serine-Arginine Splicing Factors/genetics

KW - Transcription Elongation, Genetic

KW - Transcription Termination, Genetic

U2 - 10.1016/j.cell.2019.04.038

DO - 10.1016/j.cell.2019.04.038

M3 - Journal article

C2 - 31104839

VL - 177

SP - 1797-1813.e18

JO - Cell

JF - Cell

SN - 0092-8674

IS - 7

ER -

ID: 328236328