Role of phospholipase A2 in the induction of drip loss in porcine muscle.
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Role of phospholipase A2 in the induction of drip loss in porcine muscle. / Poulsen, Kristian A; Young, Jette F; Theil, Peter; Kolko, Miriam; Oksbjerg, Niels; Lambert, Ian H.
I: Journal of Agricultural and Food Chemistry, Bind 55, Nr. 5, 2007, s. 1970-6.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Role of phospholipase A2 in the induction of drip loss in porcine muscle.
AU - Poulsen, Kristian A
AU - Young, Jette F
AU - Theil, Peter
AU - Kolko, Miriam
AU - Oksbjerg, Niels
AU - Lambert, Ian H
PY - 2007
Y1 - 2007
N2 - The role of phospholipase A2 in the induction of drip loss from pig muscle has been investigated. In samples from porcine M. longissimus dorsi, total PLA2 activity as well as mRNA and protein levels of the group VIA iPLA2 (iPLA2-VIA) increased during the initial 4 h post-mortem period. Morphological studies of porcine muscle showed that at 4 h post-mortem, gaps had formed between muscle fibers and that the sarcolemma membrane borders appeared blurred. At the same time iPLA2-VIA protein levels were increased inside muscle fibers and at the sarcolemma. iPLA2-VIA mRNA abundance in samples from different breeds of pigs with variations in drip loss revealed no clear correlation between drip loss level and iPLA2-VIA expression. Together, these data indicate that during the post-mortem period, iPLA2-VIA expression and activity is increased at the muscle fiber membranes. PLA2 activity may affect membrane permeability and consequently the progression of drip formation in porcine muscle.
AB - The role of phospholipase A2 in the induction of drip loss from pig muscle has been investigated. In samples from porcine M. longissimus dorsi, total PLA2 activity as well as mRNA and protein levels of the group VIA iPLA2 (iPLA2-VIA) increased during the initial 4 h post-mortem period. Morphological studies of porcine muscle showed that at 4 h post-mortem, gaps had formed between muscle fibers and that the sarcolemma membrane borders appeared blurred. At the same time iPLA2-VIA protein levels were increased inside muscle fibers and at the sarcolemma. iPLA2-VIA mRNA abundance in samples from different breeds of pigs with variations in drip loss revealed no clear correlation between drip loss level and iPLA2-VIA expression. Together, these data indicate that during the post-mortem period, iPLA2-VIA expression and activity is increased at the muscle fiber membranes. PLA2 activity may affect membrane permeability and consequently the progression of drip formation in porcine muscle.
U2 - 10.1021/jf062341n
DO - 10.1021/jf062341n
M3 - Journal article
C2 - 17288434
VL - 55
SP - 1970
EP - 1976
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 5
ER -
ID: 4290486