RiboMeth-seq: Profiling of 2'-O-Me in RNA

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Standard

RiboMeth-seq : Profiling of 2'-O-Me in RNA. / Krogh, Nicolai; Birkedal, Ulf; Nielsen, Henrik.

RNA Methylation. Bind 1562 Springer, 2017. s. 189-209 (Methods in Molecular Biology, Bind 1562).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Krogh, N, Birkedal, U & Nielsen, H 2017, RiboMeth-seq: Profiling of 2'-O-Me in RNA. i RNA Methylation. bind 1562, Springer, Methods in Molecular Biology, bind 1562, s. 189-209. https://doi.org/10.1007/978-1-4939-6807-7_13

APA

Krogh, N., Birkedal, U., & Nielsen, H. (2017). RiboMeth-seq: Profiling of 2'-O-Me in RNA. I RNA Methylation (Bind 1562, s. 189-209). Springer. Methods in Molecular Biology Bind 1562 https://doi.org/10.1007/978-1-4939-6807-7_13

Vancouver

Krogh N, Birkedal U, Nielsen H. RiboMeth-seq: Profiling of 2'-O-Me in RNA. I RNA Methylation. Bind 1562. Springer. 2017. s. 189-209. (Methods in Molecular Biology, Bind 1562). https://doi.org/10.1007/978-1-4939-6807-7_13

Author

Krogh, Nicolai ; Birkedal, Ulf ; Nielsen, Henrik. / RiboMeth-seq : Profiling of 2'-O-Me in RNA. RNA Methylation. Bind 1562 Springer, 2017. s. 189-209 (Methods in Molecular Biology, Bind 1562).

Bibtex

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title = "RiboMeth-seq: Profiling of 2'-O-Me in RNA",
abstract = "RiboMeth-seq is a sequencing-based method for mapping and quantitation of one of the most abundant RNA modifications, ribose methylation. It is based on a simple chemical principle, namely the several orders of magnitude difference in nucleophilicity of a 2'-OH and a 2'-O-Me. Thus, the method combines alkaline fragmentation and a specialized library construction protocol based on 5'-OH and 2',3' cyclic phosphate ends to prepare RNA for sequencing. The read-ends of library fragments are used for mapping with nucleotide resolution and calculation of the fraction of molecules methylated at the 2'-O-Me sites.",
author = "Nicolai Krogh and Ulf Birkedal and Henrik Nielsen",
year = "2017",
doi = "10.1007/978-1-4939-6807-7_13",
language = "English",
isbn = "978-1-4939-6805-3",
volume = "1562",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "189--209",
booktitle = "RNA Methylation",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - RiboMeth-seq

T2 - Profiling of 2'-O-Me in RNA

AU - Krogh, Nicolai

AU - Birkedal, Ulf

AU - Nielsen, Henrik

PY - 2017

Y1 - 2017

N2 - RiboMeth-seq is a sequencing-based method for mapping and quantitation of one of the most abundant RNA modifications, ribose methylation. It is based on a simple chemical principle, namely the several orders of magnitude difference in nucleophilicity of a 2'-OH and a 2'-O-Me. Thus, the method combines alkaline fragmentation and a specialized library construction protocol based on 5'-OH and 2',3' cyclic phosphate ends to prepare RNA for sequencing. The read-ends of library fragments are used for mapping with nucleotide resolution and calculation of the fraction of molecules methylated at the 2'-O-Me sites.

AB - RiboMeth-seq is a sequencing-based method for mapping and quantitation of one of the most abundant RNA modifications, ribose methylation. It is based on a simple chemical principle, namely the several orders of magnitude difference in nucleophilicity of a 2'-OH and a 2'-O-Me. Thus, the method combines alkaline fragmentation and a specialized library construction protocol based on 5'-OH and 2',3' cyclic phosphate ends to prepare RNA for sequencing. The read-ends of library fragments are used for mapping with nucleotide resolution and calculation of the fraction of molecules methylated at the 2'-O-Me sites.

U2 - 10.1007/978-1-4939-6807-7_13

DO - 10.1007/978-1-4939-6807-7_13

M3 - Book chapter

C2 - 28349462

SN - 978-1-4939-6805-3

VL - 1562

T3 - Methods in Molecular Biology

SP - 189

EP - 209

BT - RNA Methylation

PB - Springer

ER -

ID: 180367464