Relationship of CDK-activating kinase and RNA polymerase II CTD kinase TFIIH/TFIIK
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KIN28, a member of the p34 cdc2 CDC28 family of protein kinases, is identified as a subunit of yeast RNA polymerase transcription factor IIH (TFIIH) on the basis of sequence determination, immunological reactivity, and copurification. KIN28 is, moreover, one of three subunits of TFIIK, a subassembly of TFIIH with protein kinase activity directed toward the C-terminal repeat domain (CTD) of the largest subunit of RNA polymerase II. Itself a phosphoprotein, KIN28 interacts specifically with the two largest subunits of RNA polymerase II. Previous work of others points to two further associations: KIN28 interacts in vivo with the cyclin CCL1, and KIN28 and CCL1 are homologous to human MO15 and cyclin H, which form the cyclin-dependent kinaseactivating kinase (CAK). We show that human CAK possesses the CTD kinase activity characteristic of TFIIH.
Originalsprog | Engelsk |
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Tidsskrift | Cell |
Vol/bind | 79 |
Udgave nummer | 6 |
Sider (fra-til) | 1103-1109 |
Antal sider | 7 |
ISSN | 0092-8674 |
DOI | |
Status | Udgivet - 16 dec. 1994 |
Eksternt udgivet | Ja |
ID: 331042827