Psoralen photofootprinting of protein-binding sites on DNA
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Psoralen photofootprinting of protein-binding sites on DNA. / Zhen, W P; Jeppesen, Claus; Nielsen, Peter E.
I: FEBS Letters, Bind 229, Nr. 1, 29.02.1988, s. 73-6.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Psoralen photofootprinting of protein-binding sites on DNA
AU - Zhen, W P
AU - Jeppesen, Claus
AU - Nielsen, Peter E.
PY - 1988/2/29
Y1 - 1988/2/29
N2 - Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (OR1 operator or deo1 promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.
AB - Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (OR1 operator or deo1 promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.
KW - Animals
KW - Base Sequence
KW - Binding Sites
KW - Cattle
KW - DNA/metabolism
KW - DNA-Binding Proteins
KW - DNA-Directed RNA Polymerases/metabolism
KW - Ficusin/metabolism
KW - Furocoumarins/metabolism
KW - Molecular Sequence Data
KW - Photochemistry
KW - Protein Binding
KW - Repressor Proteins/metabolism
KW - Trioxsalen/metabolism
KW - Viral Proteins
KW - Viral Regulatory and Accessory Proteins
U2 - 10.1016/0014-5793(88)80800-7
DO - 10.1016/0014-5793(88)80800-7
M3 - Journal article
C2 - 3345840
VL - 229
SP - 73
EP - 76
JO - F E B S Letters
JF - F E B S Letters
SN - 0014-5793
IS - 1
ER -
ID: 203632482