Protoporphyrin IX formation and photobleaching in different layers of normal human skin: methyl- and hexylaminolevulinate and different light sources
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Protoporphyrin IX formation and photobleaching in different layers of normal human skin : methyl- and hexylaminolevulinate and different light sources. / Togsverd-Bo, Katrine; Idorn, Luise W; Philipsen, Peter A; Wulf, Hans Christian; Hædersdal, Merete.
I: Experimental Dermatology Online, Bind 21, Nr. 10, 2012, s. 745-750.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Protoporphyrin IX formation and photobleaching in different layers of normal human skin
T2 - methyl- and hexylaminolevulinate and different light sources
AU - Togsverd-Bo, Katrine
AU - Idorn, Luise W
AU - Philipsen, Peter A
AU - Wulf, Hans Christian
AU - Hædersdal, Merete
N1 - © 2012 John Wiley & Sons A/S.
PY - 2012
Y1 - 2012
N2 - Topical photodynamic therapy (PDT) is used for various skin disorders, and selective targeting of specific skin structures is desirable. The objective was to assess accumulation of PpIX fluorescence and photobleaching within skin layers using different photosensitizers and light sources. Normal human skin was tape-stripped and incubated with 20% methylaminolevulinate (MAL) or 20% hexylaminolevulinate (HAL) for 3 h. Fluorescence microscopy quantified PpIX accumulation in epidermis, superficial, mid and deep dermis, down to 2 mm. PpIX photobleaching by light-emitting diode (LED, 632 nm, 18 and 37 J/cm(2)), intense pulsed light (IPL, 500-650 nm, 36 and 72 J/cm(2)) and long-pulsed dye laser (LPDL, 595 nm, 7.5 and 15 J/cm(2)) was measured using fluorescence photography and microscopy. We found higher PpIX fluorescence intensities in epidermis and superficial dermis in HAL-incubated skin than MAL-incubated skin (P
AB - Topical photodynamic therapy (PDT) is used for various skin disorders, and selective targeting of specific skin structures is desirable. The objective was to assess accumulation of PpIX fluorescence and photobleaching within skin layers using different photosensitizers and light sources. Normal human skin was tape-stripped and incubated with 20% methylaminolevulinate (MAL) or 20% hexylaminolevulinate (HAL) for 3 h. Fluorescence microscopy quantified PpIX accumulation in epidermis, superficial, mid and deep dermis, down to 2 mm. PpIX photobleaching by light-emitting diode (LED, 632 nm, 18 and 37 J/cm(2)), intense pulsed light (IPL, 500-650 nm, 36 and 72 J/cm(2)) and long-pulsed dye laser (LPDL, 595 nm, 7.5 and 15 J/cm(2)) was measured using fluorescence photography and microscopy. We found higher PpIX fluorescence intensities in epidermis and superficial dermis in HAL-incubated skin than MAL-incubated skin (P
U2 - 10.1111/j.1600-0625.2012.01557.x
DO - 10.1111/j.1600-0625.2012.01557.x
M3 - Journal article
C2 - 22882358
VL - 21
SP - 745
EP - 750
JO - Experimental Dermatology
JF - Experimental Dermatology
SN - 1600-0625
IS - 10
ER -
ID: 48466151