Preservation of beta cell function in adult human pancreatic islets for several months in vitro
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Preservation of beta cell function in adult human pancreatic islets for several months in vitro. / Brunstedt, J; Andersson, A; Frimodt-Møller, C; Nielsen, Jens Høiriis.
I: Diabetologia, Bind 16, Nr. 2, 02.1979, s. 97-100.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Preservation of beta cell function in adult human pancreatic islets for several months in vitro
AU - Brunstedt, J
AU - Andersson, A
AU - Frimodt-Møller, C
AU - Nielsen, Jens Høiriis
PY - 1979/2
Y1 - 1979/2
N2 - Islets of Langerhans were isolated from four human kidney donors, aged 16 to 21 years by the collagenase method described for isolation of rodent islets. So far the human islets have been kept in tissue culture, without attachment, in medium RPMI 1640 supplemented with 10% calf serum for more than 9 months, with preservation of the ability to release insulin in response to glucose stimulation. Replacement of calf serum with serum from normal human subjects did not affect B-cell survival, but resulted in elevated insulin values partly due to lower insulin degrading activity. Thus the described technique presents a valuable tool for studying chronic effects of metabolites and hormones on islet function, as well as for islet storage prior to transplantation into humans.
AB - Islets of Langerhans were isolated from four human kidney donors, aged 16 to 21 years by the collagenase method described for isolation of rodent islets. So far the human islets have been kept in tissue culture, without attachment, in medium RPMI 1640 supplemented with 10% calf serum for more than 9 months, with preservation of the ability to release insulin in response to glucose stimulation. Replacement of calf serum with serum from normal human subjects did not affect B-cell survival, but resulted in elevated insulin values partly due to lower insulin degrading activity. Thus the described technique presents a valuable tool for studying chronic effects of metabolites and hormones on islet function, as well as for islet storage prior to transplantation into humans.
KW - Adolescent
KW - Adult
KW - Culture Media
KW - Culture Techniques
KW - Glucose
KW - Humans
KW - Insulin
KW - Islets of Langerhans
KW - Tissue Preservation
M3 - Journal article
C2 - 365659
VL - 16
SP - 97
EP - 100
JO - Diabetologia
JF - Diabetologia
SN - 0012-186X
IS - 2
ER -
ID: 47975623