Predicting the optimal STR profile amplification set up from Quantifiler™ Trio data
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Predicting the optimal STR profile amplification set up from Quantifiler™ Trio data. / Dupont, Mikkel E.; Kampmann, Marie Louise; Truelsen, Ditte M.; Petersen, Cathrine B.; Andersen, Jeppe D.
I: Forensic Science International: Genetics Supplement Series, Bind 8, 2022, s. 205-207.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning
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TY - JOUR
T1 - Predicting the optimal STR profile amplification set up from Quantifiler™ Trio data
AU - Dupont, Mikkel E.
AU - Kampmann, Marie Louise
AU - Truelsen, Ditte M.
AU - Petersen, Cathrine B.
AU - Andersen, Jeppe D.
N1 - Funding Information: The authors would like to thank René Lydeking Hansen for retrieving the data.
PY - 2022
Y1 - 2022
N2 - Samples collected for forensic case work may be of varying quality and quantity. The sample DNA is often quantified prior to short tandem repeats (STR) profile analysis with methods such as Quantifiler™Trio (QFT). The QFT measures the quantity of DNA as well as an internal PCR control (IPC) and a degradation index (DI). The aim of this study was to use IPC and DI measurements to identify samples, which would benefit from a modified PCR amplification set-up when generating the STR profiles. The sample quality of 6287 single source case work samples were categorized as 'Good’, ‘Partly degraded’, ‘Highly degraded’, ‘Inhibited’ and ‘Degraded and Inhibited’ based on the peak height ratios in the electropherogram data. The DI and IPC were correlated with the assigned quality categories of the samples. Samples categorized into the degraded and/or inhibited categories were found to have statistically significantly different DI and IPC compared to samples categorized as ‘Good’. This indicates that the additional information gained from the QFT can be useful to identify degraded and/or inhibited samples prior to the STR-profile analysis. Future work will re-evaluate the criteria of inclusion in the sample quality groups and implement multi source samples.
AB - Samples collected for forensic case work may be of varying quality and quantity. The sample DNA is often quantified prior to short tandem repeats (STR) profile analysis with methods such as Quantifiler™Trio (QFT). The QFT measures the quantity of DNA as well as an internal PCR control (IPC) and a degradation index (DI). The aim of this study was to use IPC and DI measurements to identify samples, which would benefit from a modified PCR amplification set-up when generating the STR profiles. The sample quality of 6287 single source case work samples were categorized as 'Good’, ‘Partly degraded’, ‘Highly degraded’, ‘Inhibited’ and ‘Degraded and Inhibited’ based on the peak height ratios in the electropherogram data. The DI and IPC were correlated with the assigned quality categories of the samples. Samples categorized into the degraded and/or inhibited categories were found to have statistically significantly different DI and IPC compared to samples categorized as ‘Good’. This indicates that the additional information gained from the QFT can be useful to identify degraded and/or inhibited samples prior to the STR-profile analysis. Future work will re-evaluate the criteria of inclusion in the sample quality groups and implement multi source samples.
KW - Degradation
KW - Forensic case work
KW - Inhibition
KW - Quantifiler™ Trio
KW - STR
U2 - 10.1016/j.fsigss.2022.10.036
DO - 10.1016/j.fsigss.2022.10.036
M3 - Journal article
AN - SCOPUS:85141241087
VL - 8
SP - 205
EP - 207
JO - Forensic Science International: Genetics. Supplement Series
JF - Forensic Science International: Genetics. Supplement Series
SN - 1875-1768
ER -
ID: 328528003