Plasmid transfer from Pseudomonas putida to the indigenous bacteria on alfalfa sprouts: Characterization, direct quantification, and in situ location of transconjugant cells

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Standard

Plasmid transfer from Pseudomonas putida to the indigenous bacteria on alfalfa sprouts : Characterization, direct quantification, and in situ location of transconjugant cells. / Mølbak, Lars; Licht, Tine Rask; Kvist, Thomas; Kroer, Niels; Andersen, Sigrid Rita.

I: Applied and Environmental Microbiology, Bind 69, Nr. 9, 01.09.2003, s. 5536-5542.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Mølbak, L, Licht, TR, Kvist, T, Kroer, N & Andersen, SR 2003, 'Plasmid transfer from Pseudomonas putida to the indigenous bacteria on alfalfa sprouts: Characterization, direct quantification, and in situ location of transconjugant cells', Applied and Environmental Microbiology, bind 69, nr. 9, s. 5536-5542. https://doi.org/10.1128/AEM.69.9.5536-5542.2003

APA

Mølbak, L., Licht, T. R., Kvist, T., Kroer, N., & Andersen, S. R. (2003). Plasmid transfer from Pseudomonas putida to the indigenous bacteria on alfalfa sprouts: Characterization, direct quantification, and in situ location of transconjugant cells. Applied and Environmental Microbiology, 69(9), 5536-5542. https://doi.org/10.1128/AEM.69.9.5536-5542.2003

Vancouver

Mølbak L, Licht TR, Kvist T, Kroer N, Andersen SR. Plasmid transfer from Pseudomonas putida to the indigenous bacteria on alfalfa sprouts: Characterization, direct quantification, and in situ location of transconjugant cells. Applied and Environmental Microbiology. 2003 sep. 1;69(9):5536-5542. https://doi.org/10.1128/AEM.69.9.5536-5542.2003

Author

Mølbak, Lars ; Licht, Tine Rask ; Kvist, Thomas ; Kroer, Niels ; Andersen, Sigrid Rita. / Plasmid transfer from Pseudomonas putida to the indigenous bacteria on alfalfa sprouts : Characterization, direct quantification, and in situ location of transconjugant cells. I: Applied and Environmental Microbiology. 2003 ; Bind 69, Nr. 9. s. 5536-5542.

Bibtex

@article{bb3959f9e5b14793b58b809dacd1d5c1,
title = "Plasmid transfer from Pseudomonas putida to the indigenous bacteria on alfalfa sprouts: Characterization, direct quantification, and in situ location of transconjugant cells",
abstract = "The transfer of the plasmids pJKJ5 and TOL (pWWO) from Pseudomonas putida to the indigenous bacterial community on alfalfa sprouts was studied. Tagging with fluorescent protein markers allowed direct quantification of the introduced donor bacteria and of indigenous bacteria that had received the plasmids. The sprouts were observed for 9 days; during this time alfalfa seeds, inoculated with donor bacteria, developed to edible and subsequently decaying sprouts. The first transconjugants were detected on day 6 after donor inoculation and occurred at frequencies of 3.4 × 10-4 and 2.0 × 10-6 transconjugant cells per donor cell for pKJK5::gfp and TOL::gfp, respectively. Confocal laser scanning microscopy revealed that the sprouts were heavily colonized with donors and that most transconjugants were located around the hypocotyl and root areas. Randomly selected members of the indigenous bacterial community from both inoculated and uninoculated sprouts, as well as a representative part of the community that had received the plasmids, were characterized by polymorphisms of PCR-amplified ribosomal DNA (rDNA) spacer regions between the 16S and 23S genes, followed by partial 16S rDNA sequencing. This showed that the initially dominating genera Erwinia and Paenibacillus were gradually replaced by Pseudomonas on the fully developed sprouts. Transconjugants carrying either of the investigated plasmids mainly belonged to the genera Pseudomonas and Erwinia. The numbers of transconjugant cells did not reach detectable levels until 6 days after the onset of germination, at which point these species constituted the majority of the indigenous bacteria. In conclusion, the alfalfa sprouts provided an environment that allowed noteworthy frequencies of plasmid transfer from P. putida in the absence of selective pressure that could favor the presence of the investigated plasmids.",
author = "Lars M{\o}lbak and Licht, {Tine Rask} and Thomas Kvist and Niels Kroer and Andersen, {Sigrid Rita}",
year = "2003",
month = sep,
day = "1",
doi = "10.1128/AEM.69.9.5536-5542.2003",
language = "English",
volume = "69",
pages = "5536--5542",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "9",

}

RIS

TY - JOUR

T1 - Plasmid transfer from Pseudomonas putida to the indigenous bacteria on alfalfa sprouts

T2 - Characterization, direct quantification, and in situ location of transconjugant cells

AU - Mølbak, Lars

AU - Licht, Tine Rask

AU - Kvist, Thomas

AU - Kroer, Niels

AU - Andersen, Sigrid Rita

PY - 2003/9/1

Y1 - 2003/9/1

N2 - The transfer of the plasmids pJKJ5 and TOL (pWWO) from Pseudomonas putida to the indigenous bacterial community on alfalfa sprouts was studied. Tagging with fluorescent protein markers allowed direct quantification of the introduced donor bacteria and of indigenous bacteria that had received the plasmids. The sprouts were observed for 9 days; during this time alfalfa seeds, inoculated with donor bacteria, developed to edible and subsequently decaying sprouts. The first transconjugants were detected on day 6 after donor inoculation and occurred at frequencies of 3.4 × 10-4 and 2.0 × 10-6 transconjugant cells per donor cell for pKJK5::gfp and TOL::gfp, respectively. Confocal laser scanning microscopy revealed that the sprouts were heavily colonized with donors and that most transconjugants were located around the hypocotyl and root areas. Randomly selected members of the indigenous bacterial community from both inoculated and uninoculated sprouts, as well as a representative part of the community that had received the plasmids, were characterized by polymorphisms of PCR-amplified ribosomal DNA (rDNA) spacer regions between the 16S and 23S genes, followed by partial 16S rDNA sequencing. This showed that the initially dominating genera Erwinia and Paenibacillus were gradually replaced by Pseudomonas on the fully developed sprouts. Transconjugants carrying either of the investigated plasmids mainly belonged to the genera Pseudomonas and Erwinia. The numbers of transconjugant cells did not reach detectable levels until 6 days after the onset of germination, at which point these species constituted the majority of the indigenous bacteria. In conclusion, the alfalfa sprouts provided an environment that allowed noteworthy frequencies of plasmid transfer from P. putida in the absence of selective pressure that could favor the presence of the investigated plasmids.

AB - The transfer of the plasmids pJKJ5 and TOL (pWWO) from Pseudomonas putida to the indigenous bacterial community on alfalfa sprouts was studied. Tagging with fluorescent protein markers allowed direct quantification of the introduced donor bacteria and of indigenous bacteria that had received the plasmids. The sprouts were observed for 9 days; during this time alfalfa seeds, inoculated with donor bacteria, developed to edible and subsequently decaying sprouts. The first transconjugants were detected on day 6 after donor inoculation and occurred at frequencies of 3.4 × 10-4 and 2.0 × 10-6 transconjugant cells per donor cell for pKJK5::gfp and TOL::gfp, respectively. Confocal laser scanning microscopy revealed that the sprouts were heavily colonized with donors and that most transconjugants were located around the hypocotyl and root areas. Randomly selected members of the indigenous bacterial community from both inoculated and uninoculated sprouts, as well as a representative part of the community that had received the plasmids, were characterized by polymorphisms of PCR-amplified ribosomal DNA (rDNA) spacer regions between the 16S and 23S genes, followed by partial 16S rDNA sequencing. This showed that the initially dominating genera Erwinia and Paenibacillus were gradually replaced by Pseudomonas on the fully developed sprouts. Transconjugants carrying either of the investigated plasmids mainly belonged to the genera Pseudomonas and Erwinia. The numbers of transconjugant cells did not reach detectable levels until 6 days after the onset of germination, at which point these species constituted the majority of the indigenous bacteria. In conclusion, the alfalfa sprouts provided an environment that allowed noteworthy frequencies of plasmid transfer from P. putida in the absence of selective pressure that could favor the presence of the investigated plasmids.

UR - http://www.scopus.com/inward/record.url?scp=0141481268&partnerID=8YFLogxK

U2 - 10.1128/AEM.69.9.5536-5542.2003

DO - 10.1128/AEM.69.9.5536-5542.2003

M3 - Journal article

C2 - 12957943

AN - SCOPUS:0141481268

VL - 69

SP - 5536

EP - 5542

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 9

ER -

ID: 214688532