Phylogeny and resistance profiles of HIV-1 POL sequences from rectal biopsies and blood
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Phylogeny and resistance profiles of HIV-1 POL sequences from rectal biopsies and blood. / Katzenstein, T L; Petersen, A B; Storgaard, M; Obel, N; Jensen-Fangel, Søren; Nielsen, C; Jørgensen, L B; Jørgensen, L B.
I: Journal of Medical Virology, Bind 82, Nr. 7, 01.07.2010, s. 1103-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Phylogeny and resistance profiles of HIV-1 POL sequences from rectal biopsies and blood
AU - Katzenstein, T L
AU - Petersen, A B
AU - Storgaard, M
AU - Obel, N
AU - Jensen-Fangel, Søren
AU - Nielsen, C
AU - Jørgensen, L B
AU - Jørgensen, L B
N1 - (c) 2010 Wiley-Liss, Inc.
PY - 2010/7/1
Y1 - 2010/7/1
N2 - The phylogeny and resistance profiles of human immunodeficiency virus type 1 (HIV-1) protease (PR) and reverse transcriptase (RT) sequences were compared among six patients with HIV-1 who had received numerous treatments. RNA and DNA fractions were obtained from concurrent blood and rectal biopsy samples. Phylogenetic trees and resistance profiles showed that the rectal mucosa and the peripheral blood mononuclear cells (PBMCs) harbored different HIV-1 strains. The resistance-associated mutations found in each strain corresponded to the treatment history of the patients. The resistance mutations acquired during earlier treatment regimens were detected in the sequences obtained from the rectal samples and in the PBMCs in several of the patients. Also, differences in the resistance profiles were observed between anatomical sites and between RNA and DNA fractions. Thus, a single sample probably will not be representative of the HIV-1 archived in different sites. Both the resistance profile and phylogeny of HIV-1 often differed in sequences obtained from RNA and DNA from the same site. These findings suggest that additional information regarding the antiviral resistance profile of the patient might be obtained by testing different anatomical sites.
AB - The phylogeny and resistance profiles of human immunodeficiency virus type 1 (HIV-1) protease (PR) and reverse transcriptase (RT) sequences were compared among six patients with HIV-1 who had received numerous treatments. RNA and DNA fractions were obtained from concurrent blood and rectal biopsy samples. Phylogenetic trees and resistance profiles showed that the rectal mucosa and the peripheral blood mononuclear cells (PBMCs) harbored different HIV-1 strains. The resistance-associated mutations found in each strain corresponded to the treatment history of the patients. The resistance mutations acquired during earlier treatment regimens were detected in the sequences obtained from the rectal samples and in the PBMCs in several of the patients. Also, differences in the resistance profiles were observed between anatomical sites and between RNA and DNA fractions. Thus, a single sample probably will not be representative of the HIV-1 archived in different sites. Both the resistance profile and phylogeny of HIV-1 often differed in sequences obtained from RNA and DNA from the same site. These findings suggest that additional information regarding the antiviral resistance profile of the patient might be obtained by testing different anatomical sites.
U2 - http://dx.doi.org/10.1002/jmv.21796
DO - http://dx.doi.org/10.1002/jmv.21796
M3 - Journal article
VL - 82
SP - 1103
EP - 1109
JO - Journal of Medical Virology
JF - Journal of Medical Virology
SN - 0146-6615
IS - 7
ER -
ID: 34075282