Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms
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Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms. / Nilsson, Martin; Jakobsen, Tim Holm; Givskov, Michael; Twetman, Svante; Tolker-Nielsen, Tim.
I: Microbiology, Bind 165, Nr. 3, 2019, s. 334-342.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms
AU - Nilsson, Martin
AU - Jakobsen, Tim Holm
AU - Givskov, Michael
AU - Twetman, Svante
AU - Tolker-Nielsen, Tim
PY - 2019
Y1 - 2019
N2 - Knowledge about biofilm-associated antibiotic tolerance mechanisms is warranted in order to develop effective treatments against biofilm infections. We performed a screen of a Streptococcus mutans transposon mutant library for mutants with reduced biofilm-associated antimicrobial tolerance, and found that the spxA1 gene plays a role in tolerance towards gentamicin and other antibiotics such as vancomycin and linezolid. SpxA1 is a regulator of genes involved in the oxidative stress response in S. mutans . The oxidative stress response genes gor and ahpC were found to be up-regulated upon antibiotic treatment of S. mutans wild-type biofilms, but not spxA1 mutant biofilms. The gor gene product catalyses the formation of glutathione which functions as an important antioxidant during oxidative stress, and accordingly biofilm-associated antibiotic tolerance of the spxA1 mutant could be restored by exogenous addition of glutathione. Our results indicate that the oxidative stress response plays a role in biofilm-associated antibiotic tolerance of S. mutans , and add to the on-going debate on the role of reactive oxygen species in antibiotic mediated killing of bacteria.
AB - Knowledge about biofilm-associated antibiotic tolerance mechanisms is warranted in order to develop effective treatments against biofilm infections. We performed a screen of a Streptococcus mutans transposon mutant library for mutants with reduced biofilm-associated antimicrobial tolerance, and found that the spxA1 gene plays a role in tolerance towards gentamicin and other antibiotics such as vancomycin and linezolid. SpxA1 is a regulator of genes involved in the oxidative stress response in S. mutans . The oxidative stress response genes gor and ahpC were found to be up-regulated upon antibiotic treatment of S. mutans wild-type biofilms, but not spxA1 mutant biofilms. The gor gene product catalyses the formation of glutathione which functions as an important antioxidant during oxidative stress, and accordingly biofilm-associated antibiotic tolerance of the spxA1 mutant could be restored by exogenous addition of glutathione. Our results indicate that the oxidative stress response plays a role in biofilm-associated antibiotic tolerance of S. mutans , and add to the on-going debate on the role of reactive oxygen species in antibiotic mediated killing of bacteria.
KW - Streptococcus mutans
KW - biofilm
KW - antimicrobial tolerance
KW - oxidative stress
U2 - 10.1099/mic.0.000773
DO - 10.1099/mic.0.000773
M3 - Journal article
C2 - 30663959
VL - 165
SP - 334
EP - 342
JO - Microbiology
JF - Microbiology
SN - 1350-0872
IS - 3
ER -
ID: 215968824