Novel sampling technique maintaining the two-dimensional organization of microbes during cultivation from chronic wounds: The Imprint method
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Novel sampling technique maintaining the two-dimensional organization of microbes during cultivation from chronic wounds : The Imprint method. / Iversen, Anne Kristine Servais; Fritz, Blaine Gabriel; Hansen, Mads Joachim; Kirketerp-Møller, Klaus; Jakobsen, Tim Holm; Bjarnsholt, Thomas; Lichtenberg, Mads.
I: APMIS, Bind 132, 2024, s. 210-220.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Novel sampling technique maintaining the two-dimensional organization of microbes during cultivation from chronic wounds
T2 - The Imprint method
AU - Iversen, Anne Kristine Servais
AU - Fritz, Blaine Gabriel
AU - Hansen, Mads Joachim
AU - Kirketerp-Møller, Klaus
AU - Jakobsen, Tim Holm
AU - Bjarnsholt, Thomas
AU - Lichtenberg, Mads
N1 - Publisher Copyright: © 2024 The Authors. APMIS published by John Wiley & Sons Ltd on behalf of Scandinavian Societies for Pathology, Medical Microbiology and Immunology.
PY - 2024
Y1 - 2024
N2 - This study aimed to develop and validate “the Imprint method,”, a technique for sampling microbes from chronic wounds while preserving their two-dimensional spatial organization. We used nylon filters to sample bacteria and compared with sampling using Eswabs in 12 patients. The Imprint method identified a mean of 0.93 unique species more than Eswab (4.3 ± 2.2 and 3.4 ± 1.4 unique species, respectively; mean ± SD; n = 30). Accuracy between the Eswab and the Imprint method was 93.2% and in cases of disagreement between methods, Imprint had a higher sensitivity in 6/8 of the most prevalent species. In vitro validation confirmed that the Imprint method could transfer bacterial colonies while replicating their two-dimensional organization and the area covered by bacteria on the plate sampled. Clinical testing demonstrated that the imprint method is a rapid and feasible technique that identified more unique bacterial species than Eswab with a good agreement between methods but that Imprint was better at detecting important pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa. The Imprint method is a novel technique that cultures and records the two-dimensional organization of microbes, providing an alternative or supplement to conventional surface culture using Eswab.
AB - This study aimed to develop and validate “the Imprint method,”, a technique for sampling microbes from chronic wounds while preserving their two-dimensional spatial organization. We used nylon filters to sample bacteria and compared with sampling using Eswabs in 12 patients. The Imprint method identified a mean of 0.93 unique species more than Eswab (4.3 ± 2.2 and 3.4 ± 1.4 unique species, respectively; mean ± SD; n = 30). Accuracy between the Eswab and the Imprint method was 93.2% and in cases of disagreement between methods, Imprint had a higher sensitivity in 6/8 of the most prevalent species. In vitro validation confirmed that the Imprint method could transfer bacterial colonies while replicating their two-dimensional organization and the area covered by bacteria on the plate sampled. Clinical testing demonstrated that the imprint method is a rapid and feasible technique that identified more unique bacterial species than Eswab with a good agreement between methods but that Imprint was better at detecting important pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa. The Imprint method is a novel technique that cultures and records the two-dimensional organization of microbes, providing an alternative or supplement to conventional surface culture using Eswab.
KW - bacteria
KW - chronic infection
KW - Chronic wounds
KW - culture
KW - Imprint
KW - techniques
U2 - 10.1111/apm.13372
DO - 10.1111/apm.13372
M3 - Journal article
C2 - 38270387
AN - SCOPUS:85183366423
VL - 132
SP - 210
EP - 220
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
ER -
ID: 381848061