Next-generation sequencing offers new insights into DNA degradation
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Next-generation sequencing offers new insights into DNA degradation. / Overballe-Petersen, Søren; Orlando, Ludovic Antoine Alexandre; Willerslev, Eske.
I: Trends in Biotechnology, Bind 30, Nr. 7, 2012, s. 364-368.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Next-generation sequencing offers new insights into DNA degradation
AU - Overballe-Petersen, Søren
AU - Orlando, Ludovic Antoine Alexandre
AU - Willerslev, Eske
N1 - Copyright © 2012 Elsevier Ltd. All rights reserved.
PY - 2012
Y1 - 2012
N2 - The processes underlying DNA degradation are central to various disciplines, including cancer research, forensics and archaeology. The sequencing of ancient DNA molecules on next-generation sequencing platforms provides direct measurements of cytosine deamination, depurination and fragmentation rates that previously were obtained only from extrapolations of results from in vitro kinetic experiments performed over short timescales. For example, recent next-generation sequencing of ancient DNA reveals purine bases as one of the main targets of postmortem hydrolytic damage, through base elimination and strand breakage. It also shows substantially increased rates of DNA base-loss at guanosine. In this review, we argue that the latter results from an electron resonance structure unique to guanosine rather than adenosine having an extra resonance structure over guanosine as previously suggested.
AB - The processes underlying DNA degradation are central to various disciplines, including cancer research, forensics and archaeology. The sequencing of ancient DNA molecules on next-generation sequencing platforms provides direct measurements of cytosine deamination, depurination and fragmentation rates that previously were obtained only from extrapolations of results from in vitro kinetic experiments performed over short timescales. For example, recent next-generation sequencing of ancient DNA reveals purine bases as one of the main targets of postmortem hydrolytic damage, through base elimination and strand breakage. It also shows substantially increased rates of DNA base-loss at guanosine. In this review, we argue that the latter results from an electron resonance structure unique to guanosine rather than adenosine having an extra resonance structure over guanosine as previously suggested.
KW - Animals
KW - DNA
KW - DNA Damage
KW - Fossils
KW - Horses
KW - Humans
KW - Mammals
KW - Models, Genetic
KW - Nucleic Acid Conformation
KW - Sequence Analysis, DNA
U2 - 10.1016/j.tibtech.2012.03.007
DO - 10.1016/j.tibtech.2012.03.007
M3 - Journal article
C2 - 22516743
VL - 30
SP - 364
EP - 368
JO - Trends in Biotechnology
JF - Trends in Biotechnology
SN - 0167-7799
IS - 7
ER -
ID: 49691278