Net O2 exchange rates under dark and light conditions across different stem compartments
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Net O2 exchange rates under dark and light conditions across different stem compartments. / Natale, Sara; Peralta Ogorek, Lucas Léon; Caracciolo, Ludovico; Morosinotto, Tomas; van Amerongen, Herbert; Casolo, Valentino; Pedersen, Ole; Nardini, Andrea.
I: New Phytologist, Bind 243, Nr. 1, 2024, s. 72-81.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Net O2 exchange rates under dark and light conditions across different stem compartments
AU - Natale, Sara
AU - Peralta Ogorek, Lucas Léon
AU - Caracciolo, Ludovico
AU - Morosinotto, Tomas
AU - van Amerongen, Herbert
AU - Casolo, Valentino
AU - Pedersen, Ole
AU - Nardini, Andrea
N1 - Publisher Copyright: © 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.
PY - 2024
Y1 - 2024
N2 - Woody plants display some photosynthetic activity in stems, but the biological role of stem photosynthesis and the specific contributions of bark and wood to carbon uptake and oxygen evolution remain poorly understood. We aimed to elucidate the functional characteristics of chloroplasts in stems of different ages in Fraxinus ornus. Our investigation employed diverse experimental approaches, including microsensor technology to assess oxygen production rates in whole stem, bark, and wood separately. Additionally, we utilized fluorescence lifetime imaging microscopy (FLIM) to characterize the relative abundance of photosystems I and II (PSI : PSII chlorophyll ratio) in bark and wood. Our findings revealed light-induced increases in O2 production in whole stem, bark, and wood. We present the radial profile of O2 production in F. ornus stems, demonstrating the capability of stem chloroplasts to perform light-dependent electron transport. Younger stems exhibited higher light-induced O2 production and dark respiration rates than older ones. While bark emerged as the primary contributor to net O2 production under light conditions, our data underscored that wood chloroplasts are also photosynthetically active. The FLIM analysis unveiled a lower PSI abundance in wood than in bark, suggesting stem chloroplasts are not only active but also acclimate to the spectral composition of light reaching inner compartments.
AB - Woody plants display some photosynthetic activity in stems, but the biological role of stem photosynthesis and the specific contributions of bark and wood to carbon uptake and oxygen evolution remain poorly understood. We aimed to elucidate the functional characteristics of chloroplasts in stems of different ages in Fraxinus ornus. Our investigation employed diverse experimental approaches, including microsensor technology to assess oxygen production rates in whole stem, bark, and wood separately. Additionally, we utilized fluorescence lifetime imaging microscopy (FLIM) to characterize the relative abundance of photosystems I and II (PSI : PSII chlorophyll ratio) in bark and wood. Our findings revealed light-induced increases in O2 production in whole stem, bark, and wood. We present the radial profile of O2 production in F. ornus stems, demonstrating the capability of stem chloroplasts to perform light-dependent electron transport. Younger stems exhibited higher light-induced O2 production and dark respiration rates than older ones. While bark emerged as the primary contributor to net O2 production under light conditions, our data underscored that wood chloroplasts are also photosynthetically active. The FLIM analysis unveiled a lower PSI abundance in wood than in bark, suggesting stem chloroplasts are not only active but also acclimate to the spectral composition of light reaching inner compartments.
KW - FLIM
KW - microsensor
KW - oxygen exchange rate
KW - PSI : PSII Chl ratio
KW - radial O profile
KW - stem photosynthesis
U2 - 10.1111/nph.19782
DO - 10.1111/nph.19782
M3 - Journal article
C2 - 38703003
AN - SCOPUS:85192182324
VL - 243
SP - 72
EP - 81
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 1
ER -
ID: 391676365