NBR1-mediated selective autophagy of ARF7 modulates root branching
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NBR1-mediated selective autophagy of ARF7 modulates root branching. / Ebstrup, Elise; Ansbøl, Jeppe; Paez-Garcia, Ana; Culp, Henry; Chevalier, Jonathan; Clemmens, Pauline; Coll, Núria S.; Moreno-Risueno, Miguel A.; Rodriguez, Eleazar.
I: EMBO Reports, Bind 25, 2024, s. 2571 - 2591.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - NBR1-mediated selective autophagy of ARF7 modulates root branching
AU - Ebstrup, Elise
AU - Ansbøl, Jeppe
AU - Paez-Garcia, Ana
AU - Culp, Henry
AU - Chevalier, Jonathan
AU - Clemmens, Pauline
AU - Coll, Núria S.
AU - Moreno-Risueno, Miguel A.
AU - Rodriguez, Eleazar
N1 - Publisher Copyright: © The Author(s) 2024.
PY - 2024
Y1 - 2024
N2 - Auxin dictates root architecture via the Auxin Response Factor (ARF) family of transcription factors, which control lateral root (LR) formation. In Arabidopsis, ARF7 regulates the specification of prebranch sites (PBS) generating LRs through gene expression oscillations and plays a pivotal role during LR initiation. Despite the importance of ARF7 in this process, there is a surprising lack of knowledge about how ARF7 turnover is regulated and how this impacts root architecture. Here, we show that ARF7 accumulates in autophagy mutants and is degraded through NBR1-dependent selective autophagy. We demonstrate that the previously reported rhythmic changes to ARF7 abundance in roots are modulated via autophagy and might occur in other tissues. In addition, we show that the level of co-localization between ARF7 and autophagy markers oscillates and can be modulated by auxin to trigger ARF7 turnover. Furthermore, we observe that autophagy impairment prevents ARF7 oscillation and reduces both PBS establishment and LR formation. In conclusion, we report a novel role for autophagy during development, namely by enacting auxin-induced selective degradation of ARF7 to optimize periodic root branching.
AB - Auxin dictates root architecture via the Auxin Response Factor (ARF) family of transcription factors, which control lateral root (LR) formation. In Arabidopsis, ARF7 regulates the specification of prebranch sites (PBS) generating LRs through gene expression oscillations and plays a pivotal role during LR initiation. Despite the importance of ARF7 in this process, there is a surprising lack of knowledge about how ARF7 turnover is regulated and how this impacts root architecture. Here, we show that ARF7 accumulates in autophagy mutants and is degraded through NBR1-dependent selective autophagy. We demonstrate that the previously reported rhythmic changes to ARF7 abundance in roots are modulated via autophagy and might occur in other tissues. In addition, we show that the level of co-localization between ARF7 and autophagy markers oscillates and can be modulated by auxin to trigger ARF7 turnover. Furthermore, we observe that autophagy impairment prevents ARF7 oscillation and reduces both PBS establishment and LR formation. In conclusion, we report a novel role for autophagy during development, namely by enacting auxin-induced selective degradation of ARF7 to optimize periodic root branching.
KW - ARF7
KW - Autophagy
KW - Auxin
KW - Lateral Root
KW - NBR1
U2 - 10.1038/s44319-024-00142-5
DO - 10.1038/s44319-024-00142-5
M3 - Journal article
C2 - 38684906
AN - SCOPUS:85191800461
VL - 25
SP - 2571
EP - 2591
JO - E M B O Reports
JF - E M B O Reports
SN - 1469-221X
ER -
ID: 391494009