Nanoscale alterations of corneocytes indicate skin disease
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Nanoscale alterations of corneocytes indicate skin disease. / Franz, J; Beutel, M; Gevers, K; Kramer, A; Thyssen, J P; Kezic, S; Riethmüller, C.
I: Skin Research and Technology, Bind 22, Nr. 2, 05.2016, s. 174-80.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Nanoscale alterations of corneocytes indicate skin disease
AU - Franz, J
AU - Beutel, M
AU - Gevers, K
AU - Kramer, A
AU - Thyssen, J P
AU - Kezic, S
AU - Riethmüller, C
N1 - © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
PY - 2016/5
Y1 - 2016/5
N2 - BACKGROUND: The skin barrier protects the organism against exogenous stressors and simultaneously prevents excessive water loss. While the delicate regulation of skin barrier is not completely understood, morphological and histological evaluation remain key features of clinical investigations. Here, we extended the phenotypic perspective down to the nanoscale.METHODS: Corneocyte samples were obtained non-invasively by a standard tape stripping procedure from 21 indviduals. Scanning electron (SEM) and atomic force microcopy (AFM) were used to record nanoscale topography. Circular nano-objects were identified and these were quantitated through computer vision.RESULTS: Typical dimensions of 273 nm height and 305 nm width. We showed that their density does not correlate to age or pigmentation in healthy subjects, but that they were clearly elevated in corneocytes from patients with atopic dermatitis, a common inflammatory skin condition.CONCLUSION: The presence of these corneocyte-nanostructures might be used as a diagnostic parameter for skin disorders - even in cases below a clinical threshold.
AB - BACKGROUND: The skin barrier protects the organism against exogenous stressors and simultaneously prevents excessive water loss. While the delicate regulation of skin barrier is not completely understood, morphological and histological evaluation remain key features of clinical investigations. Here, we extended the phenotypic perspective down to the nanoscale.METHODS: Corneocyte samples were obtained non-invasively by a standard tape stripping procedure from 21 indviduals. Scanning electron (SEM) and atomic force microcopy (AFM) were used to record nanoscale topography. Circular nano-objects were identified and these were quantitated through computer vision.RESULTS: Typical dimensions of 273 nm height and 305 nm width. We showed that their density does not correlate to age or pigmentation in healthy subjects, but that they were clearly elevated in corneocytes from patients with atopic dermatitis, a common inflammatory skin condition.CONCLUSION: The presence of these corneocyte-nanostructures might be used as a diagnostic parameter for skin disorders - even in cases below a clinical threshold.
KW - Adolescent
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Biomarkers
KW - Child
KW - Child, Preschool
KW - Dermatitis, Atopic
KW - Female
KW - Humans
KW - Infant
KW - Keratinocytes
KW - Male
KW - Middle Aged
KW - Nanoparticles
KW - Reproducibility of Results
KW - Sensitivity and Specificity
KW - Skin
KW - Young Adult
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1111/srt.12247
DO - 10.1111/srt.12247
M3 - Journal article
C2 - 26100642
VL - 22
SP - 174
EP - 180
JO - Skin Research and Technology
JF - Skin Research and Technology
SN - 0909-752X
IS - 2
ER -
ID: 177528532