Molecular basis for inhibition of type III-B CRISPR-Cas by an archaeal viral anti-CRISPR protein
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Molecular basis for inhibition of type III-B CRISPR-Cas by an archaeal viral anti-CRISPR protein. / Lin, Jinzhong; Alfastsen, Lauge; Bhoobalan-Chitty, Yuvaraj; Peng, Xu.
I: Cell Host and Microbe, Bind 31, Nr. 11, 2023, s. 1837-1849.e5.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Molecular basis for inhibition of type III-B CRISPR-Cas by an archaeal viral anti-CRISPR protein
AU - Lin, Jinzhong
AU - Alfastsen, Lauge
AU - Bhoobalan-Chitty, Yuvaraj
AU - Peng, Xu
N1 - Publisher Copyright: © 2023 Elsevier Inc.
PY - 2023
Y1 - 2023
N2 - Despite a wide presence of type III clustered regularly interspaced short palindromic repeats, CRISPR-associated (CRISPR-Cas) in archaea and bacteria, very few anti-CRISPR (Acr) proteins inhibiting type III immunity have been identified, and even less is known about their inhibition mechanism. Here, we present the discovery of a type III CRISPR-Cas inhibitor, AcrIIIB2, encoded by Sulfolobus virus S. islandicus rod-shaped virus 3 (SIRV3). AcrIIIB2 inhibits type III-B CRISPR-Cas immune response to protospacers encoded in middle/late-expressed viral genes. Investigation of the interactions between S. islandicus type III-B CRISPR-Cas Cmr-α-related proteins and AcrIIIB2 reveals that the Acr does not bind to Csx1 but rather interacts with the Cmr-α effector complex. Furthermore, in vitro assays demonstrate that AcrIIIB2 can block the dissociation of cleaved target RNA from the Cmr-α complex, thereby inhibiting the Cmr-α turnover, thus preventing host cellular dormancy and further viral genome degradation by the type III-B CRISPR-Cas immunity.
AB - Despite a wide presence of type III clustered regularly interspaced short palindromic repeats, CRISPR-associated (CRISPR-Cas) in archaea and bacteria, very few anti-CRISPR (Acr) proteins inhibiting type III immunity have been identified, and even less is known about their inhibition mechanism. Here, we present the discovery of a type III CRISPR-Cas inhibitor, AcrIIIB2, encoded by Sulfolobus virus S. islandicus rod-shaped virus 3 (SIRV3). AcrIIIB2 inhibits type III-B CRISPR-Cas immune response to protospacers encoded in middle/late-expressed viral genes. Investigation of the interactions between S. islandicus type III-B CRISPR-Cas Cmr-α-related proteins and AcrIIIB2 reveals that the Acr does not bind to Csx1 but rather interacts with the Cmr-α effector complex. Furthermore, in vitro assays demonstrate that AcrIIIB2 can block the dissociation of cleaved target RNA from the Cmr-α complex, thereby inhibiting the Cmr-α turnover, thus preventing host cellular dormancy and further viral genome degradation by the type III-B CRISPR-Cas immunity.
KW - Acr inhibition mechanism
KW - anti-CRISPR
KW - CRISPR-Cas turnover
KW - type III CRISPR
U2 - 10.1016/j.chom.2023.10.003
DO - 10.1016/j.chom.2023.10.003
M3 - Journal article
C2 - 37909049
AN - SCOPUS:85175531148
VL - 31
SP - 1837-1849.e5
JO - Cell Host & Microbe
JF - Cell Host & Microbe
SN - 1931-3128
IS - 11
ER -
ID: 372810596