MicroRNAs show mutually exclusive expression patterns in the brain of adult male rats
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MicroRNAs show mutually exclusive expression patterns in the brain of adult male rats. / Olsen, Line; Klausen, Mikkel; Helboe, Lone; Nielsen, Finn Cilius; Werge, Thomas; Olsen, Line; Klausen, Mikkel; Helboe, Lone; Nielsen, Finn Cilius; Werge, Thomas.
I: PLoS ONE, Bind 4, Nr. 10, 2009, s. e7225.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - MicroRNAs show mutually exclusive expression patterns in the brain of adult male rats
AU - Olsen, Line
AU - Klausen, Mikkel
AU - Helboe, Lone
AU - Nielsen, Finn Cilius
AU - Werge, Thomas
AU - Olsen, Line
AU - Klausen, Mikkel
AU - Helboe, Lone
AU - Nielsen, Finn Cilius
AU - Werge, Thomas
N1 - Keywords: Animals; Cerebellum; Cluster Analysis; Gene Expression Profiling; Gene Expression Regulation; Male; Mice; MicroRNAs; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; Prosencephalon; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, RNA
PY - 2009
Y1 - 2009
N2 - BACKGROUND: The brain is a major site of microRNA (miRNA) gene expression, but the spatial expression patterns of miRNAs within the brain have not yet been fully covered. METHODOLOGY/PRINCIPAL FINDINGS: We have characterized the regional expression profiles of miRNAs in five distinct regions of the adult rat brain: amygdala, cerebellum, hippocampus, hypothalamus and substantia nigra. Microarray profiling uncovered 48 miRNAs displaying more than three-fold enrichment between two or more brain regions. Notably, we found reciprocal expression profiles for a subset of the miRNAs predominantly found (> ten times) in either the cerebellum (miR-206 and miR-497) or the forebrain regions (miR-132, miR-212, miR-221 and miR-222). CONCLUSIONS/SIGNIFICANCE: The results indicate that some miRNAs could be important for area-specific functions in the brain. Our data, combined with previous studies in mice, provides additional guidance for future investigations of miRNA functions in the brain.
AB - BACKGROUND: The brain is a major site of microRNA (miRNA) gene expression, but the spatial expression patterns of miRNAs within the brain have not yet been fully covered. METHODOLOGY/PRINCIPAL FINDINGS: We have characterized the regional expression profiles of miRNAs in five distinct regions of the adult rat brain: amygdala, cerebellum, hippocampus, hypothalamus and substantia nigra. Microarray profiling uncovered 48 miRNAs displaying more than three-fold enrichment between two or more brain regions. Notably, we found reciprocal expression profiles for a subset of the miRNAs predominantly found (> ten times) in either the cerebellum (miR-206 and miR-497) or the forebrain regions (miR-132, miR-212, miR-221 and miR-222). CONCLUSIONS/SIGNIFICANCE: The results indicate that some miRNAs could be important for area-specific functions in the brain. Our data, combined with previous studies in mice, provides additional guidance for future investigations of miRNA functions in the brain.
U2 - 10.1371/journal.pone.0007225
DO - 10.1371/journal.pone.0007225
M3 - Journal article
C2 - 19806225
VL - 4
SP - e7225
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 10
ER -
ID: 20010895