Membrane targeting of the small GTPase Rab9 is accompanied by nucleotide exchange.
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Membrane targeting of the small GTPase Rab9 is accompanied by nucleotide exchange. / Soldati, Thierry; Shapiro, Allan D.; Svejstrup, AB; Pfeffer, SR.
I: Nature, 1994, s. 76-78.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Membrane targeting of the small GTPase Rab9 is accompanied by nucleotide exchange.
AU - Soldati, Thierry
AU - Shapiro, Allan D.
AU - Svejstrup, AB
AU - Pfeffer, SR
PY - 1994
Y1 - 1994
N2 - The Rab GTPases are key regulators of vesicular transport. A fraction of Rab proteins is present in the cytosol, bound with GDP, complexed to a protein termed GDI. Rab9 is localized primarily to late endosomes, where it aids the transport of mannose 6-phosphate receptors to the trans-Golgi network. It has been proposed that Rab proteins are delivered to specific membranes by GDI, and that this process is accompanied by the exchange of bound GDP for GTP. In addition, Rab localization requires carboxy-terminal prenylation and specific structural determinants. Here we describe the reconstitution of the selective targeting of prenylated Rab9 protein onto late endosome membranes and show that this process is accompanied by endosome-triggered nucleotide exchange.
AB - The Rab GTPases are key regulators of vesicular transport. A fraction of Rab proteins is present in the cytosol, bound with GDP, complexed to a protein termed GDI. Rab9 is localized primarily to late endosomes, where it aids the transport of mannose 6-phosphate receptors to the trans-Golgi network. It has been proposed that Rab proteins are delivered to specific membranes by GDI, and that this process is accompanied by the exchange of bound GDP for GTP. In addition, Rab localization requires carboxy-terminal prenylation and specific structural determinants. Here we describe the reconstitution of the selective targeting of prenylated Rab9 protein onto late endosome membranes and show that this process is accompanied by endosome-triggered nucleotide exchange.
U2 - 10.1038/369076a0
DO - 10.1038/369076a0
M3 - Journal article
C2 - 8164745
SP - 76
EP - 78
JO - Nature
JF - Nature
SN - 0028-0836
ER -
ID: 334652972