Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines
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- Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines
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The generation and maintenance of genome edited zebrafish lines is typically labor intensive due to the lack of an easy visual read-out for the modification. To facilitate this process, we have developed a novel method that relies on the inclusion of an artificial intron with a transgenic marker (InTraM) within theknock-insequence of interest, which upon splicing produces a transcript with a precise and seamless modification. We have demonstrated this technology by replacing the stop codon of the zebrafishfli1agene with a transcriptional activator KALTA4, using an InTraM that enables red fluorescent protein expression in the heart.
Originalsprog | Engelsk |
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Artikelnummer | 23391 |
Tidsskrift | Genesis |
Vol/bind | 58 |
Udgave nummer | 10-11 |
Antal sider | 5 |
ISSN | 1526-954X |
DOI | |
Status | Udgivet - 2020 |
ID: 280117922