In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection

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In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection. / Niola, Francesco; Dagnæs-Hansen, Frederik; Frödin, Morten.

CRISPR Gene Editing: Methods and Protocols. red. / Yonglun Luo. Bind 1961 New York, NY : Humana Press, 2019. s. 329-341 20 (Methods in molecular biology (Clifton, N.J.)).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning

Harvard

Niola, F, Dagnæs-Hansen, F & Frödin, M 2019, In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection. i Y Luo (red.), CRISPR Gene Editing: Methods and Protocols. bind 1961, 20, Humana Press, New York, NY, Methods in molecular biology (Clifton, N.J.), s. 329-341. https://doi.org/10.1007/978-1-4939-9170-9_20

APA

Niola, F., Dagnæs-Hansen, F., & Frödin, M. (2019). In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection. I Y. Luo (red.), CRISPR Gene Editing: Methods and Protocols (Bind 1961, s. 329-341). [20] Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-4939-9170-9_20

Vancouver

Niola F, Dagnæs-Hansen F, Frödin M. In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection. I Luo Y, red., CRISPR Gene Editing: Methods and Protocols. Bind 1961. New York, NY: Humana Press. 2019. s. 329-341. 20. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-4939-9170-9_20

Author

Niola, Francesco ; Dagnæs-Hansen, Frederik ; Frödin, Morten. / In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection. CRISPR Gene Editing: Methods and Protocols. red. / Yonglun Luo. Bind 1961 New York, NY : Humana Press, 2019. s. 329-341 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{0ae21ad26dd640768725904dd3a089e6,
title = "In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection",
abstract = "CRISPR/Cas9 technology allows facile modification of the genome in virtually any desired way through the use of easily designed plasmid constructs that express a gRNA targeting a genomic site-of-interest and Cas9. Hydrodynamic tail vein injection, on the other hand, is a simple method to deliver {"}naked{"} plasmid DNA to 5-40% of the hepatocytes of the liver of adult mice. Here, we describe how these two techniques can be combined to create a workflow for fast, easy, and cost-efficient in vivo genome editing of the adult mouse liver. Using this method, large cohorts of mice with genetically modified livers can be established within 3 weeks to generate models for gene function in normal physiology and diseases of the liver.",
keywords = "Animals, CRISPR-Cas Systems/genetics, Gene Editing, Hepatocytes/metabolism, Liver/metabolism, Mice, Plasmids/genetics, RNA, Guide/genetics",
author = "Francesco Niola and Frederik Dagn{\ae}s-Hansen and Morten Fr{\"o}din",
year = "2019",
doi = "10.1007/978-1-4939-9170-9_20",
language = "English",
isbn = "978-1-4939-9169-3",
volume = "1961",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "329--341",
editor = "Yonglun Luo",
booktitle = "CRISPR Gene Editing",
address = "United States",

}

RIS

TY - CHAP

T1 - In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection

AU - Niola, Francesco

AU - Dagnæs-Hansen, Frederik

AU - Frödin, Morten

PY - 2019

Y1 - 2019

N2 - CRISPR/Cas9 technology allows facile modification of the genome in virtually any desired way through the use of easily designed plasmid constructs that express a gRNA targeting a genomic site-of-interest and Cas9. Hydrodynamic tail vein injection, on the other hand, is a simple method to deliver "naked" plasmid DNA to 5-40% of the hepatocytes of the liver of adult mice. Here, we describe how these two techniques can be combined to create a workflow for fast, easy, and cost-efficient in vivo genome editing of the adult mouse liver. Using this method, large cohorts of mice with genetically modified livers can be established within 3 weeks to generate models for gene function in normal physiology and diseases of the liver.

AB - CRISPR/Cas9 technology allows facile modification of the genome in virtually any desired way through the use of easily designed plasmid constructs that express a gRNA targeting a genomic site-of-interest and Cas9. Hydrodynamic tail vein injection, on the other hand, is a simple method to deliver "naked" plasmid DNA to 5-40% of the hepatocytes of the liver of adult mice. Here, we describe how these two techniques can be combined to create a workflow for fast, easy, and cost-efficient in vivo genome editing of the adult mouse liver. Using this method, large cohorts of mice with genetically modified livers can be established within 3 weeks to generate models for gene function in normal physiology and diseases of the liver.

KW - Animals

KW - CRISPR-Cas Systems/genetics

KW - Gene Editing

KW - Hepatocytes/metabolism

KW - Liver/metabolism

KW - Mice

KW - Plasmids/genetics

KW - RNA, Guide/genetics

U2 - 10.1007/978-1-4939-9170-9_20

DO - 10.1007/978-1-4939-9170-9_20

M3 - Book chapter

C2 - 30912055

SN - 978-1-4939-9169-3

VL - 1961

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 329

EP - 341

BT - CRISPR Gene Editing

A2 - Luo, Yonglun

PB - Humana Press

CY - New York, NY

ER -

ID: 231415213