In Vitro Maturation and Culture of Human Oocytes
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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In Vitro Maturation and Culture of Human Oocytes. / Gruhn, Jennifer R.; Kristensen, Stine Gry; Andersen, Claus Yding; Hoffmann, Eva R.
Mouse Oocyte Development: Methods and Protocols. red. / Marie-Hélène Verlhac; Marie-Emilie Terret. Humana Press, 2018. s. 23-30 (Methods in Molecular Biology, Bind 1818).Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - In Vitro Maturation and Culture of Human Oocytes
AU - Gruhn, Jennifer R.
AU - Kristensen, Stine Gry
AU - Andersen, Claus Yding
AU - Hoffmann, Eva R.
PY - 2018
Y1 - 2018
N2 - We describe the collection, culture, and ex vivo, in vitro maturation of germinal vesicle (GV) oocytes obtained from human small antral follicles (hSAFs). hSAFs contain fully grown GV oocytes and have the advantages that they are more numerous than large or mature follicles, which are used in IVF treatment. hSAFs can be obtained directly from human ovarian tissue without exogenous gonadotrophin stimulation and therefore allows studies of oocytes even from young women and girls. The method described here was developed to study human female meiosis but could in theory also be used for fertility treatment.
AB - We describe the collection, culture, and ex vivo, in vitro maturation of germinal vesicle (GV) oocytes obtained from human small antral follicles (hSAFs). hSAFs contain fully grown GV oocytes and have the advantages that they are more numerous than large or mature follicles, which are used in IVF treatment. hSAFs can be obtained directly from human ovarian tissue without exogenous gonadotrophin stimulation and therefore allows studies of oocytes even from young women and girls. The method described here was developed to study human female meiosis but could in theory also be used for fertility treatment.
KW - Aneuploidy
KW - Cumulus oocyte complex
KW - Germinal vesicle
KW - Human small antral follicles
KW - In vitro maturation
KW - Meiosis
KW - Metaphase II
U2 - 10.1007/978-1-4939-8603-3_3
DO - 10.1007/978-1-4939-8603-3_3
M3 - Book chapter
C2 - 29961251
AN - SCOPUS:85049440353
SN - 978-1-4939-8602-6
T3 - Methods in Molecular Biology
SP - 23
EP - 30
BT - Mouse Oocyte Development
A2 - Verlhac, Marie-Hélène
A2 - Terret, Marie-Emilie
PB - Humana Press
ER -
ID: 201674852