TY - JOUR

T1 - Immunophenotypical characterization of human neutrophil differentiation

AU - Jensen, Helena Mora

AU - Jendholm, Johan

AU - Fossum, Anna

AU - Porse, Bo

AU - Borregaad, Niels

AU - Theilgaard-Mönch, Kim

PY - 2011/6/7

Y1 - 2011/6/7

N2 - The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocyte. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.

AB - The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocyte. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.

U2 - 10.1189/jlb.0311123

DO - 10.1189/jlb.0311123

M3 - Journal article

C2 - 21653237

SP - 629

EP - 634

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

ER -