Immunomicroscopic localization of the 10,000 molecular weight calcium-binding protein in the human enterocyte
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Immunomicroscopic localization of the 10,000 molecular weight calcium-binding protein in the human enterocyte. / Staun, M; Friis, S; Dabelsteen, E; Hansen, Gert Helge.
I: Acta Pathologica Microbiologica et Immunologica Scandinavica, Bind 97, Nr. 10, 1989, s. 901-7.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Immunomicroscopic localization of the 10,000 molecular weight calcium-binding protein in the human enterocyte
AU - Staun, M
AU - Friis, S
AU - Dabelsteen, E
AU - Hansen, Gert Helge
N1 - Keywords: Calcium-Binding Proteins; Fluorescent Antibody Technique; Gold; Humans; Immunohistochemistry; Jejunum; Microscopy, Electron; Molecular Weight
PY - 1989
Y1 - 1989
N2 - The cellular localization of the 10,000 molecular weight calcium-binding protein (CaBP or Calbindin-D) in the small intestinal epithelium of man was investigated by immunofluorescence and immunoelectron microscopy (immunogold staining). Indirect immunofluorescence on frozen sections showed intracellular staining in the enterocyte. The fluorescence was evenly distributed and no significant differences were observed between crypt and villus cells. No staining was found in goblet cells or in the submucosa. Correspondingly, immunogold labeled antibodies were scattered over the cytoplasm of the enterocyte. The terminal region appeared to be the most intensely decorated and the brush border region showed labeling above the background level. No labeling was associated with intracellular membranes or the basolateral membrane.
AB - The cellular localization of the 10,000 molecular weight calcium-binding protein (CaBP or Calbindin-D) in the small intestinal epithelium of man was investigated by immunofluorescence and immunoelectron microscopy (immunogold staining). Indirect immunofluorescence on frozen sections showed intracellular staining in the enterocyte. The fluorescence was evenly distributed and no significant differences were observed between crypt and villus cells. No staining was found in goblet cells or in the submucosa. Correspondingly, immunogold labeled antibodies were scattered over the cytoplasm of the enterocyte. The terminal region appeared to be the most intensely decorated and the brush border region showed labeling above the background level. No labeling was associated with intracellular membranes or the basolateral membrane.
M3 - Journal article
C2 - 2679808
VL - 97
SP - 901
EP - 907
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 10
ER -
ID: 9748970