Human platelet lysate improves the growth and survival of cultured human pre-antral follicles
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Dokumenter
- 1-s2.0-S1472648323003589-main
Forlagets udgivne version, 1,98 MB, PDF-dokument
How do platelet-rich plasma products like human platelet lysate (HPL) and umbilical cord plasma (UCP) affect the growth and survival of isolated human pre-antral follicles in vitro?
Design
Human pre-antral follicles (n = 724; mean diameter: 75 µm; range: 46–237 µm) were isolated from ovarian medulla donated by 14 patients undergoing unilateral oophorectomy for ovarian tissue cryopreservation. Follicles were encapsulated in 0.5% alginate and cultured for 8 days in media supplemented with 5% fetal bovine serum (FBS) (n = 171), 2.5% human serum albumin (HSA) (n = 159), 5% HPL (n = 223) or 5% UCP (n = 171).
Results
The survival probability was significantly higher in the group supplemented with HPL (80%) compared with the other three groups: FBS (54%, P < 0.001); HSA (63%, P = 0.004) and UCP (29%, P < 0.001). Surviving follicles in the UCP group had less defined follicular membranes and decompacted granulosa cell layers. The median growth of surviving follicles was significantly (P < 0.001) larger in the HPL group (73 µm) compared with any of the other three groups: HSA (43 μm); FBS (40 μm) UCP (54 μm). A descriptive analysis of follicular secretion of anti-Müllerian hormone and oestradiol did not reveal any difference between the groups. The detectability of follicular genes was high for AR (100%), AMHR2 (100%) and FSHR (76%), whereas few follicles expressed LHR (20%).
Conclusion
Human platelet lysate significantly improved survival and growth of cultured human pre-antral follicles compared with FBS, HSA and UCP. The use of HPL is a valuable improvement to culture human pre-antral follicles but further studies will have to prove whether the superiority of HPL translates into better quality oocytes.
Originalsprog | Engelsk |
---|---|
Artikelnummer | 103256 |
Tidsskrift | Reproductive BioMedicine Online |
Vol/bind | 47 |
Udgave nummer | 5 |
Antal sider | 12 |
ISSN | 1472-6483 |
DOI | |
Status | Udgivet - 2023 |
Bibliografisk note
Funding Information:
This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement, number 860960.
Funding Information:
The financial support from MSCA ITN No 860960 EUROVA is gratefully acknowledged. The authors thank all members of the Laboratory of Reproductive Biology for their help, critical comments and specially Marjo Westerdahl for the technical assistance. Thanks to all the patients who donated their medulla tissue for research and to all personnel involved in the clinical activities in the ovarian tissue cryopreservation program in Denmark.
Publisher Copyright:
© 2023 The Author(s)
Antal downloads er baseret på statistik fra Google Scholar og www.ku.dk
ID: 371126040