Human blood eosinophils produce and secrete interleukin 4
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Human blood eosinophils produce and secrete interleukin 4. / Bjerke, T.; Gaustadnes, M.; Nielsen, S.; Nielsen, L. P.; Schiøtz, P. O.; Rudiger, N.; Reimert, C. M.; Dahl, R.; Christensen, I.; Poulsen, L. K.
I: Respiratory Medicine, Bind 90, Nr. 5, 1996, s. 271-277.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Human blood eosinophils produce and secrete interleukin 4
AU - Bjerke, T.
AU - Gaustadnes, M.
AU - Nielsen, S.
AU - Nielsen, L. P.
AU - Schiøtz, P. O.
AU - Rudiger, N.
AU - Reimert, C. M.
AU - Dahl, R.
AU - Christensen, I.
AU - Poulsen, L. K.
N1 - Funding Information: This study was supported by grants from National-foreningens Forsknings fond, the Novo-Nordisk Foundation, the Biotechnology Center for Signalling Peptides, the Biomembrane Research Center, University of Aarhus and Institute of Experimental Clinical Research, University Hospital of Aarhus.
PY - 1996
Y1 - 1996
N2 - Interleukin 4 (Il-4) is an immunoregulatory cytokine which induces T-cell proliferation and differentiation into a Th2 phenotype, and is of particular importance for the induction of IgE synthesis. In the present study, the capability of human peripheral blood eosinophils from allergic and non-allergic donors to produce Il-4 was examined. Using reverse transcribed polymerase chain reaction (RT-PCR), it was shown that highly purified eosinophils from allergic patients express mRNA for Il-4. Resting eosinophils also gave specific immunoreactivity with anti-Il-4 antibodies, consistent with translation of Il-4 mRNA. Light and electron microscopic immunocytochemistry revealed that Il-4 was prestored in the eosinophilic granules. These results were confirmed by Il-4 specific ELISA which showed that Il-4 production could be upregulated in the eosinophils and released from the eosinophils following stimulation with the calcium ionophore A23187. These data indicate that eosinophils may be an important source of Il-4 at sites of allergic inflammation. Thus, eosinophils may act as immunomodulatory cells enhancing the allergic response through formation of Th2-cells and inducing the isotype switching to IgE in human B-cells.
AB - Interleukin 4 (Il-4) is an immunoregulatory cytokine which induces T-cell proliferation and differentiation into a Th2 phenotype, and is of particular importance for the induction of IgE synthesis. In the present study, the capability of human peripheral blood eosinophils from allergic and non-allergic donors to produce Il-4 was examined. Using reverse transcribed polymerase chain reaction (RT-PCR), it was shown that highly purified eosinophils from allergic patients express mRNA for Il-4. Resting eosinophils also gave specific immunoreactivity with anti-Il-4 antibodies, consistent with translation of Il-4 mRNA. Light and electron microscopic immunocytochemistry revealed that Il-4 was prestored in the eosinophilic granules. These results were confirmed by Il-4 specific ELISA which showed that Il-4 production could be upregulated in the eosinophils and released from the eosinophils following stimulation with the calcium ionophore A23187. These data indicate that eosinophils may be an important source of Il-4 at sites of allergic inflammation. Thus, eosinophils may act as immunomodulatory cells enhancing the allergic response through formation of Th2-cells and inducing the isotype switching to IgE in human B-cells.
UR - http://www.scopus.com/inward/record.url?scp=15844381713&partnerID=8YFLogxK
U2 - 10.1016/S0954-6111(96)90098-0
DO - 10.1016/S0954-6111(96)90098-0
M3 - Journal article
C2 - 9499811
AN - SCOPUS:15844381713
VL - 90
SP - 271
EP - 277
JO - Respiratory Medicine
JF - Respiratory Medicine
SN - 0954-6111
IS - 5
ER -
ID: 313375168