HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells.
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HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells. / Olsen, Jørgen; Laustsen, Lotte; Troelsen, J.
I: FEBS Letters, Bind 342, Nr. 3, 1994, s. 325-8.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells.
AU - Olsen, Jørgen
AU - Laustsen, Lotte
AU - Troelsen, J
N1 - Keywords: Aminopeptidases; Animals; Antigens, CD13; Cell Nucleus; DNA-Binding Proteins; Gene Expression Regulation, Neoplastic; Hela Cells; Hepatocyte Nuclear Factor 1; Hepatocyte Nuclear Factor 1-alpha; Hepatocyte Nuclear Factor 1-beta; Humans; Intestinal Mucosa; Nuclear Proteins; Promoter Regions (Genetics); Protein Binding; Swine; Transcription Factors; Tumor Cells, Cultured
PY - 1994
Y1 - 1994
N2 - The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation of the promoter by HNF1 alpha through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.
AB - The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation of the promoter by HNF1 alpha through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.
M3 - Journal article
C2 - 7908650
VL - 342
SP - 325
EP - 328
JO - F E B S Letters
JF - F E B S Letters
SN - 0014-5793
IS - 3
ER -
ID: 6586636