Generation of induced pluripotent stem cells (iPSC) from an atrial fibrillation patient carrying a PITX2 p.M200V mutation
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Atrial fibrillation (AF) is the most common sustained arrhythmia associated with several cardiac risk factors, but increasing evidences indicated a genetic component. Indeed, genetic variations of the specific PITX2 gene have been identified in patients with early-onset AF. To investigate the molecular mechanisms underlying AF, we reprogrammed to pluripotency polymorphonucleated leukocytes isolated from the blood of a patient carrying a PITX2 p.M200V mutation, using a commercially available non-integrating expression system. The generated iPSCs expressed pluripotency markers and differentiated toward cells belonging to the three embryonic germ layers. Moreover, the cells showed a normal karyotype and retained the PITX2 p.M200V mutation.
Originalsprog | Engelsk |
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Tidsskrift | Stem Cell Research |
Vol/bind | 24 |
Sider (fra-til) | 8-11 |
Antal sider | 4 |
ISSN | 1873-5061 |
DOI | |
Status | Udgivet - okt. 2017 |
Eksternt udgivet | Ja |
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ID: 196039542