Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

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Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene. / Kaucher, Amy V.

I: Scientific Reports, 2017.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Kaucher, AV 2017, 'Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene.', Scientific Reports. https://doi.org/10.1038/srep40176

APA

Kaucher, A. V. (2017). Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene. Scientific Reports. https://doi.org/10.1038/srep40176

Vancouver

Kaucher AV. Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene. Scientific Reports. 2017. https://doi.org/10.1038/srep40176

Author

Kaucher, Amy V. / Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene. I: Scientific Reports. 2017.

Bibtex

@article{817b04f89eb64469ac2843da536ca571,
title = "Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene.",
abstract = "Genome editing tools have revolutionized the generation of genetically modified animals including livestock. In particular, the domestic pig is a proven model of human physiology and an agriculturally important species. In this study, we utilized the CRISPR/Cas9 system to edit the NANOS2 gene in pig embryos to generate offspring with mono-allelic and bi-allelic mutations. We found that NANOS2 knockout pigs phenocopy knockout mice with male specific germline ablation but other aspects of testicular development are normal. Moreover, male pigs with one intact NANOS2 allele and female knockout pigs are fertile. From an agriculture perspective, NANOS2 knockout male pigs are expected to serve as an ideal surrogate for transplantation of donor spermatogonial stem cells to expand the availability of gametes from genetically desirable sires.",
author = "Kaucher, {Amy V}",
year = "2017",
doi = "10.1038/srep40176",
language = "English",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene.

AU - Kaucher, Amy V

PY - 2017

Y1 - 2017

N2 - Genome editing tools have revolutionized the generation of genetically modified animals including livestock. In particular, the domestic pig is a proven model of human physiology and an agriculturally important species. In this study, we utilized the CRISPR/Cas9 system to edit the NANOS2 gene in pig embryos to generate offspring with mono-allelic and bi-allelic mutations. We found that NANOS2 knockout pigs phenocopy knockout mice with male specific germline ablation but other aspects of testicular development are normal. Moreover, male pigs with one intact NANOS2 allele and female knockout pigs are fertile. From an agriculture perspective, NANOS2 knockout male pigs are expected to serve as an ideal surrogate for transplantation of donor spermatogonial stem cells to expand the availability of gametes from genetically desirable sires.

AB - Genome editing tools have revolutionized the generation of genetically modified animals including livestock. In particular, the domestic pig is a proven model of human physiology and an agriculturally important species. In this study, we utilized the CRISPR/Cas9 system to edit the NANOS2 gene in pig embryos to generate offspring with mono-allelic and bi-allelic mutations. We found that NANOS2 knockout pigs phenocopy knockout mice with male specific germline ablation but other aspects of testicular development are normal. Moreover, male pigs with one intact NANOS2 allele and female knockout pigs are fertile. From an agriculture perspective, NANOS2 knockout male pigs are expected to serve as an ideal surrogate for transplantation of donor spermatogonial stem cells to expand the availability of gametes from genetically desirable sires.

U2 - 10.1038/srep40176

DO - 10.1038/srep40176

M3 - Journal article

C2 - 28071690

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

ER -

ID: 301735064