TY - JOUR

T1 - From plant scent defense to biopesticide discovery

T2 - Evaluation of toxicity and acetylcholinesterase docking properties for Lamiaceae monoterpenes

AU - de Sena Filho, Jose Guedes

AU - Soares de Almeida, Anderson

AU - Pinto-Zevallos, Delia

AU - Barreto, Ighor Costa

AU - Cabral de Holanda Cavalcanti, Sócrates

AU - Nunes, Rogeria

AU - Teodoro, Adenir Vieira

AU - Xavier, Haroudo Sátiro

AU - Barbosa Filho, José Maria

AU - Guan, Leluo

AU - Neves, Andre L.A.

AU - Duringer, Jennifer M.

N1 - Publisher Copyright: © 2022 Elsevier Ltd

PY - 2023

Y1 - 2023

N2 - Monoterpenes are a highly diverse group of chemical scents that originate from plant secondary metabolic processes, one purpose of which is to serve as a defense against herbivores. Plant-derived monoterpenes produced from Lamiaceae species have demonstrated acaricidal activity against pest mites and ticks. However, the mechanism by which these compounds carry out the demise of this group of arachnids is poorly understood. Here, we review the chemistry and bioactivity of these promising compounds and subsequently assess their potential toxicity to mites and ticks through measurement of their docking ability to amino acid residues of the binding pocket of Drosophila melanogaster acetylcholinesterase (AChE). We identified 27 monoterpenes of the Lamiaceae family from the literature that were effective against mite (Varroidae, Tetranychidae, Eriophyidae) and tick (Ixodidae) species that are problematic in agricultural production. Screening of these compounds showed that monoterpenoids possessing methyl groups, such as carvacrol, linalool, α-terpineol, bornyl acetate, and terpine-4-ol, strongly bind to D. melanogaster AChE. Linalool, which fits into the binding pocket in the amino acid catalytic triad of AChE (oxyanion hole residues, hydrogen bond interaction with GLU 237, and anionic binding with TRP 83), was identified as the most promising target for further optimization studies. We propose that monoterpenes which interact strongly with amino acid residues of the AChE receptor be targeted for development of effective, naturally produced biocontrol pesticides, as this model demonstrates potential for discovery of new acaricide compounds in a high throughput manner.

AB - Monoterpenes are a highly diverse group of chemical scents that originate from plant secondary metabolic processes, one purpose of which is to serve as a defense against herbivores. Plant-derived monoterpenes produced from Lamiaceae species have demonstrated acaricidal activity against pest mites and ticks. However, the mechanism by which these compounds carry out the demise of this group of arachnids is poorly understood. Here, we review the chemistry and bioactivity of these promising compounds and subsequently assess their potential toxicity to mites and ticks through measurement of their docking ability to amino acid residues of the binding pocket of Drosophila melanogaster acetylcholinesterase (AChE). We identified 27 monoterpenes of the Lamiaceae family from the literature that were effective against mite (Varroidae, Tetranychidae, Eriophyidae) and tick (Ixodidae) species that are problematic in agricultural production. Screening of these compounds showed that monoterpenoids possessing methyl groups, such as carvacrol, linalool, α-terpineol, bornyl acetate, and terpine-4-ol, strongly bind to D. melanogaster AChE. Linalool, which fits into the binding pocket in the amino acid catalytic triad of AChE (oxyanion hole residues, hydrogen bond interaction with GLU 237, and anionic binding with TRP 83), was identified as the most promising target for further optimization studies. We propose that monoterpenes which interact strongly with amino acid residues of the AChE receptor be targeted for development of effective, naturally produced biocontrol pesticides, as this model demonstrates potential for discovery of new acaricide compounds in a high throughput manner.

KW - Acaricide

KW - Essential oil

KW - Mites

KW - monoterpenes

KW - Ticks

U2 - 10.1016/j.cropro.2022.106126

DO - 10.1016/j.cropro.2022.106126

M3 - Review

AN - SCOPUS:85141227562

VL - 164

JO - Crop Protection

JF - Crop Protection

SN - 0261-2194

M1 - 106126

ER -