TY - JOUR

T1 - Fluorescence Screening of DNA-AgNCs with Pulsed White Light Excitation

AU - Liisberg, Mikkel Baldtzer

AU - Vosch, Tom

N1 - Funding Information: M.B.L. and T.V. acknowledge funding from the Villum Foundation (VKR023115) and the Independent Research Fund Denmark (0136-00024B). Publisher Copyright: © 2024 The Authors. Published by American Chemical Society.

PY - 2024

Y1 - 2024

N2 - DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of fluorophores with interesting photophysical properties dominated by the choice of DNA sequence. Screening methods with ultraviolet excitation and steady state well plate readers have previously been used for deepening the understanding between DNA sequence and emission color of the resulting DNA-AgNCs. Here, we present a new method for screening DNA-AgNCs by using pulsed white light excitation (λex ≈ 490-900 nm). By subtraction and time gating we are able to circumvent the dominating scatter of the white excitation light and extract both temporally and spectrally resolved emission of DNA-AgNCs over the visible to near-infrared range. Additionally, we are able to identify weak long-lived emission, which is often buried underneath the intense nanosecond fluorescence. This new approach will be useful for future screening of DNA-AgNCs (or other novel emissive materials) and aid machine-learning models by providing a richer training data set.

AB - DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of fluorophores with interesting photophysical properties dominated by the choice of DNA sequence. Screening methods with ultraviolet excitation and steady state well plate readers have previously been used for deepening the understanding between DNA sequence and emission color of the resulting DNA-AgNCs. Here, we present a new method for screening DNA-AgNCs by using pulsed white light excitation (λex ≈ 490-900 nm). By subtraction and time gating we are able to circumvent the dominating scatter of the white excitation light and extract both temporally and spectrally resolved emission of DNA-AgNCs over the visible to near-infrared range. Additionally, we are able to identify weak long-lived emission, which is often buried underneath the intense nanosecond fluorescence. This new approach will be useful for future screening of DNA-AgNCs (or other novel emissive materials) and aid machine-learning models by providing a richer training data set.

KW - DNA-AgNCs

KW - Dual Emission

KW - Fluorescence Screening

KW - TCSPC

KW - TWINS

KW - White Light Excitation

U2 - 10.1021/acs.nanolett.4c01561

DO - 10.1021/acs.nanolett.4c01561

M3 - Journal article

C2 - 38905483

AN - SCOPUS:85196907957

VL - 24

SP - 7987

EP - 7991

JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 26

ER -