Expression of IGF-II mRNA-binding proteins (IMPs) in gonads and testicular cancer
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Expression of IGF-II mRNA-binding proteins (IMPs) in gonads and testicular cancer. / Hammer, Niels A; Hansen, Thomas v O; Byskov, Anne Grete; Rajpert-De Meyts, Eva; Grøndahl, Marie Louise; Bredkjaer, Helle E; Wewer, Ulla M; Christiansen, Jan; Nielsen, Finn C.
I: Reproduction, Bind 130, Nr. 2, 2005, s. 203-12.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Expression of IGF-II mRNA-binding proteins (IMPs) in gonads and testicular cancer
AU - Hammer, Niels A
AU - Hansen, Thomas v O
AU - Byskov, Anne Grete
AU - Rajpert-De Meyts, Eva
AU - Grøndahl, Marie Louise
AU - Bredkjaer, Helle E
AU - Wewer, Ulla M
AU - Christiansen, Jan
AU - Nielsen, Finn C
PY - 2005
Y1 - 2005
N2 - Insulin-like growth factor-II mRNA-binding proteins 1, 2 and 3 (IMP1, IMP2 and IMP3) belong to a family of RNA-binding proteins implicated in mRNA localization, turnover and translational control. We examined their expression pattern during development of murine and human testis and ovaries. In the mouse, IMPs were expressed in male and female gonadal cells at embryonic day 12.5 (E12.5). From E16.5, IMP1 and IMP3 became restricted to the developing germ cells, whereas IMP2 expression persisted in the interstitial cells. In mature mouse and human ovaries, IMP1, IMP2 and IMP3 were detected in resting and growing oocytes and in the granulosa cells. In testis, IMP1 and IMP3 were found mainly in the spermatogonia, whereas IMP2 was expressed in the immature Leydig cells. Moreover, all three IMPs were detected in human semen. The developmental expression pattern of IMP1 and IMP3 in the human testis prompted us to examine their possible involvement in testicular neoplasia. IMPs were detected primarily in germ-cell neoplasms, including preinvasive testicular carcinoma in situ, classical and spermatocytic seminoma, and nonseminomas, with particularly high expression in undifferentiated embryonal carcinoma. The relative expression of IMP1, IMP2 and IMP3 varied among tumor types and only IMP1 was detected in all carcinoma in situ cells. Thus IMPs, and in particular IMP1, may be useful auxiliary markers of testicular neoplasia.
AB - Insulin-like growth factor-II mRNA-binding proteins 1, 2 and 3 (IMP1, IMP2 and IMP3) belong to a family of RNA-binding proteins implicated in mRNA localization, turnover and translational control. We examined their expression pattern during development of murine and human testis and ovaries. In the mouse, IMPs were expressed in male and female gonadal cells at embryonic day 12.5 (E12.5). From E16.5, IMP1 and IMP3 became restricted to the developing germ cells, whereas IMP2 expression persisted in the interstitial cells. In mature mouse and human ovaries, IMP1, IMP2 and IMP3 were detected in resting and growing oocytes and in the granulosa cells. In testis, IMP1 and IMP3 were found mainly in the spermatogonia, whereas IMP2 was expressed in the immature Leydig cells. Moreover, all three IMPs were detected in human semen. The developmental expression pattern of IMP1 and IMP3 in the human testis prompted us to examine their possible involvement in testicular neoplasia. IMPs were detected primarily in germ-cell neoplasms, including preinvasive testicular carcinoma in situ, classical and spermatocytic seminoma, and nonseminomas, with particularly high expression in undifferentiated embryonal carcinoma. The relative expression of IMP1, IMP2 and IMP3 varied among tumor types and only IMP1 was detected in all carcinoma in situ cells. Thus IMPs, and in particular IMP1, may be useful auxiliary markers of testicular neoplasia.
KW - Animals
KW - Blastocyst
KW - Blotting, Western
KW - Carcinoma in Situ
KW - Carcinoma, Embryonal
KW - Embryonic Development
KW - Female
KW - Gestational Age
KW - Gonads
KW - Granulosa Cells
KW - Humans
KW - Immunohistochemistry
KW - Insulin-Like Growth Factor II
KW - Leydig Cells
KW - Male
KW - Mice
KW - Neoplasm Proteins
KW - Neoplasms, Germ Cell and Embryonal
KW - Oocytes
KW - RNA-Binding Proteins
KW - Semen
KW - Seminoma
KW - Spermatozoa
KW - Testicular Neoplasms
KW - Testis
KW - Tumor Markers, Biological
U2 - 10.1530/rep.1.00664
DO - 10.1530/rep.1.00664
M3 - Journal article
C2 - 16049158
VL - 130
SP - 203
EP - 212
JO - Reproduction
JF - Reproduction
SN - 1470-1626
IS - 2
ER -
ID: 86905