Endomucin marks quiescent long-term multi-lineage repopulating hematopoietic stem cells and is essential for their transendothelial migration

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Endomucin marks quiescent long-term multi-lineage repopulating hematopoietic stem cells and is essential for their transendothelial migration. / Engelhard, Sophia; Estruch, Montserrat; Qin, Shuyu; Engelhard, Christoph A.; Rodriguez-Gonzalez, Francisco G.; Drilsvik, Martine; Martin-Gonzalez, Javier; Lu, Jeng Wei; Bryder, David; Nerlov, Claus; Weischenfeldt, Joachim; Reckzeh, Kristian; Theilgaard-Mönch, Kim.

I: Cell Reports, Bind 43, Nr. 7, 114475, 2024.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Engelhard, S, Estruch, M, Qin, S, Engelhard, CA, Rodriguez-Gonzalez, FG, Drilsvik, M, Martin-Gonzalez, J, Lu, JW, Bryder, D, Nerlov, C, Weischenfeldt, J, Reckzeh, K & Theilgaard-Mönch, K 2024, 'Endomucin marks quiescent long-term multi-lineage repopulating hematopoietic stem cells and is essential for their transendothelial migration', Cell Reports, bind 43, nr. 7, 114475. https://doi.org/10.1016/j.celrep.2024.114475

APA

Engelhard, S., Estruch, M., Qin, S., Engelhard, C. A., Rodriguez-Gonzalez, F. G., Drilsvik, M., Martin-Gonzalez, J., Lu, J. W., Bryder, D., Nerlov, C., Weischenfeldt, J., Reckzeh, K., & Theilgaard-Mönch, K. (2024). Endomucin marks quiescent long-term multi-lineage repopulating hematopoietic stem cells and is essential for their transendothelial migration. Cell Reports, 43(7), [114475]. https://doi.org/10.1016/j.celrep.2024.114475

Vancouver

Engelhard S, Estruch M, Qin S, Engelhard CA, Rodriguez-Gonzalez FG, Drilsvik M o.a. Endomucin marks quiescent long-term multi-lineage repopulating hematopoietic stem cells and is essential for their transendothelial migration. Cell Reports. 2024;43(7). 114475. https://doi.org/10.1016/j.celrep.2024.114475

Author

Engelhard, Sophia ; Estruch, Montserrat ; Qin, Shuyu ; Engelhard, Christoph A. ; Rodriguez-Gonzalez, Francisco G. ; Drilsvik, Martine ; Martin-Gonzalez, Javier ; Lu, Jeng Wei ; Bryder, David ; Nerlov, Claus ; Weischenfeldt, Joachim ; Reckzeh, Kristian ; Theilgaard-Mönch, Kim. / Endomucin marks quiescent long-term multi-lineage repopulating hematopoietic stem cells and is essential for their transendothelial migration. I: Cell Reports. 2024 ; Bind 43, Nr. 7.

Bibtex

@article{1e46d238822c4ad2ad7956bc30f0e71e,

title = "Endomucin marks quiescent long-term multi-lineage repopulating hematopoietic stem cells and is essential for their transendothelial migration",

abstract = "Endomucin (EMCN) currently represents the only hematopoietic stem cell (HSC) marker expressed by both murine and human HSCs. Here, we report that EMCN+ long-term repopulating HSCs (LT-HSCs; CD150+CD48−LSK) have a higher long-term multi-lineage repopulating capacity compared to EMCN− LT-HSCs. Cell cycle analyses and transcriptional profiling demonstrated that EMCN+ LT-HSCs were more quiescent compared to EMCN− LT-HSCs. Emcn−/− and Emcn+/+ mice displayed comparable steady-state hematopoiesis, as well as frequencies, transcriptional programs, and long-term multi-lineage repopulating capacity of their LT-HSCs. Complementary functional analyses further revealed increased cell cycle entry upon treatment with 5-fluorouracil and reduced granulocyte colony-stimulating factor (GCSF) mobilization of Emcn−/− LT-HSCs, demonstrating that EMCN expression by LT-HSCs associates with quiescence in response to hematopoietic stress and is indispensable for effective LT-HSC mobilization. Transplantation of wild-type bone marrow cells into Emcn−/− or Emcn+/+ recipients demonstrated that EMCN is essential for endothelial cell-dependent maintenance/self-renewal of the LT-HSC pool and sustained blood cell production post-transplant.",

keywords = "bone marrow niche, CP: Stem cell research, cross-species HSC marker, endomucin, endothelial cells, hematopoietic stem cell, mobilization, transplantation",

author = "Sophia Engelhard and Montserrat Estruch and Shuyu Qin and Engelhard, {Christoph A.} and Rodriguez-Gonzalez, {Francisco G.} and Martine Drilsvik and Javier Martin-Gonzalez and Lu, {Jeng Wei} and David Bryder and Claus Nerlov and Joachim Weischenfeldt and Kristian Reckzeh and Kim Theilgaard-M{\"o}nch",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors",

year = "2024",

doi = "10.1016/j.celrep.2024.114475",

language = "English",

volume = "43",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "7",

}

RIS

TY - JOUR

T1 - Endomucin marks quiescent long-term multi-lineage repopulating hematopoietic stem cells and is essential for their transendothelial migration

AU - Engelhard, Sophia

AU - Estruch, Montserrat

AU - Qin, Shuyu

AU - Engelhard, Christoph A.

AU - Rodriguez-Gonzalez, Francisco G.

AU - Drilsvik, Martine

AU - Martin-Gonzalez, Javier

AU - Lu, Jeng Wei

AU - Bryder, David

AU - Nerlov, Claus

AU - Weischenfeldt, Joachim

AU - Reckzeh, Kristian

AU - Theilgaard-Mönch, Kim

PY - 2024

Y1 - 2024

N2 - Endomucin (EMCN) currently represents the only hematopoietic stem cell (HSC) marker expressed by both murine and human HSCs. Here, we report that EMCN+ long-term repopulating HSCs (LT-HSCs; CD150+CD48−LSK) have a higher long-term multi-lineage repopulating capacity compared to EMCN− LT-HSCs. Cell cycle analyses and transcriptional profiling demonstrated that EMCN+ LT-HSCs were more quiescent compared to EMCN− LT-HSCs. Emcn−/− and Emcn+/+ mice displayed comparable steady-state hematopoiesis, as well as frequencies, transcriptional programs, and long-term multi-lineage repopulating capacity of their LT-HSCs. Complementary functional analyses further revealed increased cell cycle entry upon treatment with 5-fluorouracil and reduced granulocyte colony-stimulating factor (GCSF) mobilization of Emcn−/− LT-HSCs, demonstrating that EMCN expression by LT-HSCs associates with quiescence in response to hematopoietic stress and is indispensable for effective LT-HSC mobilization. Transplantation of wild-type bone marrow cells into Emcn−/− or Emcn+/+ recipients demonstrated that EMCN is essential for endothelial cell-dependent maintenance/self-renewal of the LT-HSC pool and sustained blood cell production post-transplant.

AB - Endomucin (EMCN) currently represents the only hematopoietic stem cell (HSC) marker expressed by both murine and human HSCs. Here, we report that EMCN+ long-term repopulating HSCs (LT-HSCs; CD150+CD48−LSK) have a higher long-term multi-lineage repopulating capacity compared to EMCN− LT-HSCs. Cell cycle analyses and transcriptional profiling demonstrated that EMCN+ LT-HSCs were more quiescent compared to EMCN− LT-HSCs. Emcn−/− and Emcn+/+ mice displayed comparable steady-state hematopoiesis, as well as frequencies, transcriptional programs, and long-term multi-lineage repopulating capacity of their LT-HSCs. Complementary functional analyses further revealed increased cell cycle entry upon treatment with 5-fluorouracil and reduced granulocyte colony-stimulating factor (GCSF) mobilization of Emcn−/− LT-HSCs, demonstrating that EMCN expression by LT-HSCs associates with quiescence in response to hematopoietic stress and is indispensable for effective LT-HSC mobilization. Transplantation of wild-type bone marrow cells into Emcn−/− or Emcn+/+ recipients demonstrated that EMCN is essential for endothelial cell-dependent maintenance/self-renewal of the LT-HSC pool and sustained blood cell production post-transplant.

KW - bone marrow niche

KW - CP: Stem cell research

KW - cross-species HSC marker

KW - endomucin

KW - endothelial cells

KW - hematopoietic stem cell

KW - mobilization

KW - transplantation

U2 - 10.1016/j.celrep.2024.114475

DO - 10.1016/j.celrep.2024.114475

M3 - Journal article

C2 - 38996072

AN - SCOPUS:85198069529

VL - 43

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 7

M1 - 114475

ER -

ID: 399235369