Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false-negative test results
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Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene : be alert of false-negative test results. / Jørgensen, Rikke Lind; Lerche, Christian Johann; Pedersen, Martin Schou; Kirkby, Nikolai Soren; Botnen, Amanda Bolt; Trebbien, Ramona; Nilsson-Møller, Stephen; Pinholt, Mette; Nielsen, Alex Christian Yde; Westh, Henrik; Lisby, Jan Gorm; Schneider, Uffe Vest.
I: APMIS, Bind 130, Nr. 10, 2022, s. 612-617.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene
T2 - be alert of false-negative test results
AU - Jørgensen, Rikke Lind
AU - Lerche, Christian Johann
AU - Pedersen, Martin Schou
AU - Kirkby, Nikolai Soren
AU - Botnen, Amanda Bolt
AU - Trebbien, Ramona
AU - Nilsson-Møller, Stephen
AU - Pinholt, Mette
AU - Nielsen, Alex Christian Yde
AU - Westh, Henrik
AU - Lisby, Jan Gorm
AU - Schneider, Uffe Vest
N1 - Publisher Copyright: © 2022 The Authors. APMIS published by John Wiley & Sons Ltd on behalf of Scandinavian Societies for Medical Microbiology and Pathology.
PY - 2022
Y1 - 2022
N2 - In March 2022, we observed samples with a negative fluorescent signal (60.5%, n = 43) for the influenza A matrix gene and a stronger positive signal for subtype A(H3N2). Forty-three samples were positive in InfA (H3N2) (mean Cq 30.9, range 23.9–35.1), and 26 of the 43 samples were negative in InfA matrix (mean Cq 28.0, range 23.2–30.6). Our multiplex test is a laboratory-developed four-target, four-color influenza A reverse-transcription PCR assay targeting the matrix gene, subtypes A(H3N2) and A(H1N1)pdm09. Several samples were negative when retested on commercial influenza Point-of-Care assays. As the matrix gene is a stand-alone target in most commercial diagnostic assays, we caution against false-negative subtype A test results.
AB - In March 2022, we observed samples with a negative fluorescent signal (60.5%, n = 43) for the influenza A matrix gene and a stronger positive signal for subtype A(H3N2). Forty-three samples were positive in InfA (H3N2) (mean Cq 30.9, range 23.9–35.1), and 26 of the 43 samples were negative in InfA matrix (mean Cq 28.0, range 23.2–30.6). Our multiplex test is a laboratory-developed four-target, four-color influenza A reverse-transcription PCR assay targeting the matrix gene, subtypes A(H3N2) and A(H1N1)pdm09. Several samples were negative when retested on commercial influenza Point-of-Care assays. As the matrix gene is a stand-alone target in most commercial diagnostic assays, we caution against false-negative subtype A test results.
KW - Assay
KW - diagnostic
KW - genetic drift
KW - H3N2
KW - M gene
KW - mutations
KW - RT-PCR
KW - sequencing
KW - surveillance
U2 - 10.1111/apm.13262
DO - 10.1111/apm.13262
M3 - Journal article
C2 - 35836366
AN - SCOPUS:85135608051
VL - 130
SP - 612
EP - 617
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 10
ER -
ID: 320505134