Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip

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Standard

Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip. / Selmeczi, David; Hansen, Thomas S; Met, Ozcan; Svane, Inge Marie; Larsen, Niels B.

I: Biomedical Microdevices, Bind 13, Nr. 2, 04.2011, s. 383-92.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Selmeczi, D, Hansen, TS, Met, O, Svane, IM & Larsen, NB 2011, 'Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip', Biomedical Microdevices, bind 13, nr. 2, s. 383-92. https://doi.org/10.1007/s10544-010-9507-1

APA

Selmeczi, D., Hansen, T. S., Met, O., Svane, I. M., & Larsen, N. B. (2011). Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip. Biomedical Microdevices, 13(2), 383-92. https://doi.org/10.1007/s10544-010-9507-1

Vancouver

Selmeczi D, Hansen TS, Met O, Svane IM, Larsen NB. Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip. Biomedical Microdevices. 2011 apr.;13(2):383-92. https://doi.org/10.1007/s10544-010-9507-1

Author

Selmeczi, David ; Hansen, Thomas S ; Met, Ozcan ; Svane, Inge Marie ; Larsen, Niels B. / Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip. I: Biomedical Microdevices. 2011 ; Bind 13, Nr. 2. s. 383-92.

Bibtex

@article{ca8d5502c79247d8bde7a3d02c5c547c,

title = "Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip",

abstract = "We present a hybrid chip of polymer and stainless steel designed for high-throughput continuous electroporation of cells in suspension. The chip is constructed with two parallel stainless steel mesh electrodes oriented perpendicular to the liquid flow. The relatively high hydrodynamic resistance of the micrometer sized holes in the meshes compared to the main channel enforces an almost homogeneous flow velocity between the meshes. Thereby, very uniform electroporation of the cells can be accomplished. Successful electroporation of 20 million human dendritic cells with mRNA is demonstrated. The performance of the chip is similar to that of the traditional electroporation cuvette, but without an upper limit on the number of cells to be electroporated. The device is constructed with two female Luer parts and can easily be integrated with other microfluidic components. Furthermore it is fabricated from injection molded polymer parts and commercially available stainless steel mesh, making it suitable for inexpensive mass production.",

keywords = "Dendritic Cells, Disposable Equipment, Electroporation, Green Fluorescent Proteins, Humans, Kinetics, Microfluidic Analytical Techniques, Polymers, RNA, Messenger, Reproducibility of Results, Stainless Steel, Transfection, Journal Article",

author = "David Selmeczi and Hansen, {Thomas S} and Ozcan Met and Svane, {Inge Marie} and Larsen, {Niels B}",

year = "2011",

month = apr,

doi = "10.1007/s10544-010-9507-1",

language = "English",

volume = "13",

pages = "383--92",

journal = "Biomedical Microdevices",

issn = "1387-2176",

publisher = "Springer",

number = "2",

}

RIS

TY - JOUR

T1 - Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip

AU - Selmeczi, David

AU - Hansen, Thomas S

AU - Met, Ozcan

AU - Svane, Inge Marie

AU - Larsen, Niels B

PY - 2011/4

Y1 - 2011/4

N2 - We present a hybrid chip of polymer and stainless steel designed for high-throughput continuous electroporation of cells in suspension. The chip is constructed with two parallel stainless steel mesh electrodes oriented perpendicular to the liquid flow. The relatively high hydrodynamic resistance of the micrometer sized holes in the meshes compared to the main channel enforces an almost homogeneous flow velocity between the meshes. Thereby, very uniform electroporation of the cells can be accomplished. Successful electroporation of 20 million human dendritic cells with mRNA is demonstrated. The performance of the chip is similar to that of the traditional electroporation cuvette, but without an upper limit on the number of cells to be electroporated. The device is constructed with two female Luer parts and can easily be integrated with other microfluidic components. Furthermore it is fabricated from injection molded polymer parts and commercially available stainless steel mesh, making it suitable for inexpensive mass production.

AB - We present a hybrid chip of polymer and stainless steel designed for high-throughput continuous electroporation of cells in suspension. The chip is constructed with two parallel stainless steel mesh electrodes oriented perpendicular to the liquid flow. The relatively high hydrodynamic resistance of the micrometer sized holes in the meshes compared to the main channel enforces an almost homogeneous flow velocity between the meshes. Thereby, very uniform electroporation of the cells can be accomplished. Successful electroporation of 20 million human dendritic cells with mRNA is demonstrated. The performance of the chip is similar to that of the traditional electroporation cuvette, but without an upper limit on the number of cells to be electroporated. The device is constructed with two female Luer parts and can easily be integrated with other microfluidic components. Furthermore it is fabricated from injection molded polymer parts and commercially available stainless steel mesh, making it suitable for inexpensive mass production.

KW - Dendritic Cells

KW - Disposable Equipment

KW - Electroporation

KW - Green Fluorescent Proteins

KW - Humans

KW - Kinetics

KW - Microfluidic Analytical Techniques

KW - Polymers

KW - RNA, Messenger

KW - Reproducibility of Results

KW - Stainless Steel

KW - Transfection

KW - Journal Article

U2 - 10.1007/s10544-010-9507-1

DO - 10.1007/s10544-010-9507-1

M3 - Journal article

C2 - 21207149

VL - 13

SP - 383

EP - 392

JO - Biomedical Microdevices

JF - Biomedical Microdevices

SN - 1387-2176

IS - 2

ER -

ID: 184774219