Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry

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Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry. / Bassani-Sternberg, Michal; Bräunlein, Eva; Klar, Richard; Engleitner, Thomas; Sinitcyn, Pavel; Audehm, Stefan; Straub, Melanie; Weber, Julia; Slotta-Huspenina, Julia; Specht, Katja; Martignoni, Marc E; Werner, Angelika; Hein, Rüdiger; H Busch, Dirk; Peschel, Christian; Rad, Roland; Cox, Jürgen; Mann, Matthias; Krackhardt, Angela M.

I: Nature Communications, Bind 7, 21.11.2016, s. 13404.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Bassani-Sternberg, M, Bräunlein, E, Klar, R, Engleitner, T, Sinitcyn, P, Audehm, S, Straub, M, Weber, J, Slotta-Huspenina, J, Specht, K, Martignoni, ME, Werner, A, Hein, R, H Busch, D, Peschel, C, Rad, R, Cox, J, Mann, M & Krackhardt, AM 2016, 'Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry', Nature Communications, bind 7, s. 13404. https://doi.org/10.1038/ncomms13404

APA

Bassani-Sternberg, M., Bräunlein, E., Klar, R., Engleitner, T., Sinitcyn, P., Audehm, S., Straub, M., Weber, J., Slotta-Huspenina, J., Specht, K., Martignoni, M. E., Werner, A., Hein, R., H Busch, D., Peschel, C., Rad, R., Cox, J., Mann, M., & Krackhardt, A. M. (2016). Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry. Nature Communications, 7, 13404. https://doi.org/10.1038/ncomms13404

Vancouver

Bassani-Sternberg M, Bräunlein E, Klar R, Engleitner T, Sinitcyn P, Audehm S o.a. Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry. Nature Communications. 2016 nov. 21;7:13404. https://doi.org/10.1038/ncomms13404

Author

Bassani-Sternberg, Michal ; Bräunlein, Eva ; Klar, Richard ; Engleitner, Thomas ; Sinitcyn, Pavel ; Audehm, Stefan ; Straub, Melanie ; Weber, Julia ; Slotta-Huspenina, Julia ; Specht, Katja ; Martignoni, Marc E ; Werner, Angelika ; Hein, Rüdiger ; H Busch, Dirk ; Peschel, Christian ; Rad, Roland ; Cox, Jürgen ; Mann, Matthias ; Krackhardt, Angela M. / Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry. I: Nature Communications. 2016 ; Bind 7. s. 13404.

Bibtex

@article{31076e0862bb43ffb7df2b538564605a,
title = "Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry",
abstract = "Although mutations may represent attractive targets for immunotherapy, direct identification of mutated peptide ligands isolated from human leucocyte antigens (HLA) on the surface of native tumour tissue has so far not been successful. Using advanced mass spectrometry (MS) analysis, we survey the melanoma-associated immunopeptidome to a depth of 95,500 patient-presented peptides. We thereby discover a large spectrum of attractive target antigen candidates including cancer testis antigens and phosphopeptides. Most importantly, we identify peptide ligands presented on native tumour tissue samples harbouring somatic mutations. Four of eleven mutated ligands prove to be immunogenic by neoantigen-specific T-cell responses. Moreover, tumour-reactive T cells with specificity for selected neoantigens identified by MS are detected in the patient's tumour and peripheral blood. We conclude that direct identification of mutated peptide ligands from primary tumour material by MS is possible and yields true neoepitopes with high relevance for immunotherapeutic strategies in cancer.",
keywords = "Journal Article",
author = "Michal Bassani-Sternberg and Eva Br{\"a}unlein and Richard Klar and Thomas Engleitner and Pavel Sinitcyn and Stefan Audehm and Melanie Straub and Julia Weber and Julia Slotta-Huspenina and Katja Specht and Martignoni, {Marc E} and Angelika Werner and R{\"u}diger Hein and {H Busch}, Dirk and Christian Peschel and Roland Rad and J{\"u}rgen Cox and Matthias Mann and Krackhardt, {Angela M}",
year = "2016",
month = nov,
day = "21",
doi = "10.1038/ncomms13404",
language = "English",
volume = "7",
pages = "13404",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry

AU - Bassani-Sternberg, Michal

AU - Bräunlein, Eva

AU - Klar, Richard

AU - Engleitner, Thomas

AU - Sinitcyn, Pavel

AU - Audehm, Stefan

AU - Straub, Melanie

AU - Weber, Julia

AU - Slotta-Huspenina, Julia

AU - Specht, Katja

AU - Martignoni, Marc E

AU - Werner, Angelika

AU - Hein, Rüdiger

AU - H Busch, Dirk

AU - Peschel, Christian

AU - Rad, Roland

AU - Cox, Jürgen

AU - Mann, Matthias

AU - Krackhardt, Angela M

PY - 2016/11/21

Y1 - 2016/11/21

N2 - Although mutations may represent attractive targets for immunotherapy, direct identification of mutated peptide ligands isolated from human leucocyte antigens (HLA) on the surface of native tumour tissue has so far not been successful. Using advanced mass spectrometry (MS) analysis, we survey the melanoma-associated immunopeptidome to a depth of 95,500 patient-presented peptides. We thereby discover a large spectrum of attractive target antigen candidates including cancer testis antigens and phosphopeptides. Most importantly, we identify peptide ligands presented on native tumour tissue samples harbouring somatic mutations. Four of eleven mutated ligands prove to be immunogenic by neoantigen-specific T-cell responses. Moreover, tumour-reactive T cells with specificity for selected neoantigens identified by MS are detected in the patient's tumour and peripheral blood. We conclude that direct identification of mutated peptide ligands from primary tumour material by MS is possible and yields true neoepitopes with high relevance for immunotherapeutic strategies in cancer.

AB - Although mutations may represent attractive targets for immunotherapy, direct identification of mutated peptide ligands isolated from human leucocyte antigens (HLA) on the surface of native tumour tissue has so far not been successful. Using advanced mass spectrometry (MS) analysis, we survey the melanoma-associated immunopeptidome to a depth of 95,500 patient-presented peptides. We thereby discover a large spectrum of attractive target antigen candidates including cancer testis antigens and phosphopeptides. Most importantly, we identify peptide ligands presented on native tumour tissue samples harbouring somatic mutations. Four of eleven mutated ligands prove to be immunogenic by neoantigen-specific T-cell responses. Moreover, tumour-reactive T cells with specificity for selected neoantigens identified by MS are detected in the patient's tumour and peripheral blood. We conclude that direct identification of mutated peptide ligands from primary tumour material by MS is possible and yields true neoepitopes with high relevance for immunotherapeutic strategies in cancer.

KW - Journal Article

U2 - 10.1038/ncomms13404

DO - 10.1038/ncomms13404

M3 - Journal article

C2 - 27869121

VL - 7

SP - 13404

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

ER -

ID: 184324084