Dimethyl sulfoxide decreases the fluorescence yield of the reaction between histamine and ortho-phthalaldehyde and may influence histamine release
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Dimethyl sulfoxide decreases the fluorescence yield of the reaction between histamine and ortho-phthalaldehyde and may influence histamine release. / Moldt, P.; Andersen, W. K.; Brøgger Christensen, S.
I: Agents and Actions, Bind 24, Nr. 1-2, 01.06.1988, s. 35-39.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Dimethyl sulfoxide decreases the fluorescence yield of the reaction between histamine and ortho-phthalaldehyde and may influence histamine release
AU - Moldt, P.
AU - Andersen, W. K.
AU - Brøgger Christensen, S.
PY - 1988/6/1
Y1 - 1988/6/1
N2 - A sensitive method for quantifying histamine is the assay based on the fluorescence of the product obtained after reacting the amine with ortho-phthalaldehyde. The presence of dimethyl sulfoxide (DMSO) even in concentrations as low as 1% decreases the yield of fluorescence. Neglecting this quenching leads to the erroneous conclusion that DMSO in a concentration dependent manner inhibits the thapsigargin induced histamine release from peritoneal rat mast cells. Apart from the release induced by the selective calcium ion mobilizers thapsigargin and ionophore A23147, DMSO potently inhibits the response to other secretagogues.
AB - A sensitive method for quantifying histamine is the assay based on the fluorescence of the product obtained after reacting the amine with ortho-phthalaldehyde. The presence of dimethyl sulfoxide (DMSO) even in concentrations as low as 1% decreases the yield of fluorescence. Neglecting this quenching leads to the erroneous conclusion that DMSO in a concentration dependent manner inhibits the thapsigargin induced histamine release from peritoneal rat mast cells. Apart from the release induced by the selective calcium ion mobilizers thapsigargin and ionophore A23147, DMSO potently inhibits the response to other secretagogues.
UR - http://www.scopus.com/inward/record.url?scp=0023896320&partnerID=8YFLogxK
U2 - 10.1007/BF01968077
DO - 10.1007/BF01968077
M3 - Journal article
C2 - 2457301
AN - SCOPUS:0023896320
VL - 24
SP - 35
EP - 39
JO - Agents and Actions
JF - Agents and Actions
SN - 0065-4299
IS - 1-2
ER -
ID: 232599319