Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum
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- acsomega.8b00424
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Antigen recognition by antibodies plays an important role in human biology and in the development of diseases. This interaction provides a basis for multiple diagnostic assays and is a guide for treatments. We have developed dihydropyridine-based fluorophores that form stable complexes with double-stranded DNA and upon recognition of the antibodies to DNA (anti-DNA) provide an optical response. The fluorophores described herein have advantageous optical properties compared to those of the currently available dyes making them valuable for research and clinical diagnostics. By studying a series of novel fluorophores, crucial parameters for the design were established, providing the required sensitivity and specificity in the detection of antibodies. Using these DNA-fluorophore complexes in a direct immunofluorescence assay, antibodies to DNA are specifically detected in 80 patients diagnosed with an autoimmune disease, systemic lupus erythematosus. Positivity indicated by emission change of α-(4′-O-methoxyphenyl)-2-furyl dihydropyridine strongly correlates with other disease biomarkers and autoimmune arthritis.
Originalsprog | Engelsk |
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Tidsskrift | ACS Omega |
Vol/bind | 3 |
Udgave nummer | 7 |
Sider (fra-til) | 7580-7586 |
Antal sider | 7 |
ISSN | 2470-1343 |
DOI | |
Status | Udgivet - 31 jul. 2018 |
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