TY - JOUR

T1 - Diagnostic accuracy of EUS-guided through-the-needle-biopsies and simultaneously obtained fine needle aspiration for cytology from pancreatic cysts

T2 - A systematic review and meta-analysis

AU - Rift, Charlotte Vestrup

AU - Scheie, David

AU - Toxværd, Anders

AU - Kovacevic, Bojan

AU - Klausen, Pia

AU - Vilmann, Peter

AU - Hansen, Carsten Palnæs

AU - Lund, Eva Løbner

AU - Hasselby, Jane Preuss

N1 - Publisher Copyright: © 2021 Elsevier GmbH

PY - 2021

Y1 - 2021

N2 - Objectives: To address the diagnostic accuracy of endoscopic ultrasound guided through-the-needle-biopsies (TTNBs) and simultaneously obtained cytology samples from pancreatic cysts compared to the final histopathological diagnosis of the surgical specimen, and to give an overview of ancillary tests performed on TTNBs. Methods: A literature search was conducted in MEDLINE, Embase and Scopus. Studies were included in the meta-analysis, if they had data for TTNB, cytology and a surgical specimen of pancreatic cysts as reference standard. The assessment of the risk of bias and quality of the included studies was conducted using the modified QUADAS-2 tool. Results: Ten studies with 99 patients were included in the meta-analysis. Data regarding study design and clinicopathological features were extracted systematically. For TTNB, pooled sensitivity was 0.86 (95 % CI 0.62−0.96), specificity 0.95 (95 % CI 0.79−0.99) and area under the curve (AUC) 0.86 for the diagnosis of a mucinous cyst and pooled sensitivity was 0.78 (95 % CI 0.61−0.89), specificity 0.99 (95 % CI 0.90−0.99) and AUC 0.92 for the diagnosis of a high-risk cyst. For a specific diagnosis, pooled sensitivity was 0.69 (95 % CI 0.50−0.83), specificity 0.47 (95 % CI 0.28−0.68) and AUC 0.49. For cytology performed simultaneously, pooled sensitivity was 0.46 (95 % CI 0.35−0.57), specificity 0.90 (95 % CI 0.46−0.99) and AUC 0.64 for the diagnosis of mucinous cysts, and pooled sensitivity was 0.38 (95 % CI 0.23−0.55), specificity 0.99 (95 % CI 0.90−0.99) and AUC 0.84 for the diagnosis of a high-risk cyst. For a specific diagnosis, pooled sensitivity was 0.29 (95 % CI 0.21−0.39), specificity 0.45 (95 % CI 0.25−0.66) and AUC 0.30. Furthermore, immunohistochemical stains can be useful to establish the specific cyst subtype. Conclusions: TTNBs have a higher sensitivity and specificity than cytology for the diagnosis of mucinous cyst and high- risk cysts of the pancreas.

AB - Objectives: To address the diagnostic accuracy of endoscopic ultrasound guided through-the-needle-biopsies (TTNBs) and simultaneously obtained cytology samples from pancreatic cysts compared to the final histopathological diagnosis of the surgical specimen, and to give an overview of ancillary tests performed on TTNBs. Methods: A literature search was conducted in MEDLINE, Embase and Scopus. Studies were included in the meta-analysis, if they had data for TTNB, cytology and a surgical specimen of pancreatic cysts as reference standard. The assessment of the risk of bias and quality of the included studies was conducted using the modified QUADAS-2 tool. Results: Ten studies with 99 patients were included in the meta-analysis. Data regarding study design and clinicopathological features were extracted systematically. For TTNB, pooled sensitivity was 0.86 (95 % CI 0.62−0.96), specificity 0.95 (95 % CI 0.79−0.99) and area under the curve (AUC) 0.86 for the diagnosis of a mucinous cyst and pooled sensitivity was 0.78 (95 % CI 0.61−0.89), specificity 0.99 (95 % CI 0.90−0.99) and AUC 0.92 for the diagnosis of a high-risk cyst. For a specific diagnosis, pooled sensitivity was 0.69 (95 % CI 0.50−0.83), specificity 0.47 (95 % CI 0.28−0.68) and AUC 0.49. For cytology performed simultaneously, pooled sensitivity was 0.46 (95 % CI 0.35−0.57), specificity 0.90 (95 % CI 0.46−0.99) and AUC 0.64 for the diagnosis of mucinous cysts, and pooled sensitivity was 0.38 (95 % CI 0.23−0.55), specificity 0.99 (95 % CI 0.90−0.99) and AUC 0.84 for the diagnosis of a high-risk cyst. For a specific diagnosis, pooled sensitivity was 0.29 (95 % CI 0.21−0.39), specificity 0.45 (95 % CI 0.25−0.66) and AUC 0.30. Furthermore, immunohistochemical stains can be useful to establish the specific cyst subtype. Conclusions: TTNBs have a higher sensitivity and specificity than cytology for the diagnosis of mucinous cyst and high- risk cysts of the pancreas.

KW - Cyst

KW - Cytology

KW - Diagnostic test

KW - Meta-analysis

KW - Pancreas

KW - Through-the-needle-biopsy

U2 - 10.1016/j.prp.2021.153368

DO - 10.1016/j.prp.2021.153368

M3 - Review

C2 - 33652239

AN - SCOPUS:85101651371

VL - 220

JO - Pathology, Research and Practice

JF - Pathology, Research and Practice

SN - 0344-0338

M1 - 153368

ER -