Comparison of techniques for quantification of next-generation sequencing libraries
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Comparison of techniques for quantification of next-generation sequencing libraries. / Hussing, Christian; Kampmann, Marie-Louise; Mogensen, Helle Smidt; Børsting, Claus; Morling, Niels.
I: Forensic Science International: Genetics. Supplement Series, Bind 5, 12.2015, s. e276-e278.Publikation: Bidrag til tidsskrift › Konferenceartikel › Forskning › fagfællebedømt
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TY - GEN
T1 - Comparison of techniques for quantification of next-generation sequencing libraries
AU - Hussing, Christian
AU - Kampmann, Marie-Louise
AU - Mogensen, Helle Smidt
AU - Børsting, Claus
AU - Morling, Niels
PY - 2015/12
Y1 - 2015/12
N2 - To ensure efficient sequencing, the DNA of next-generation sequencing (NGS) libraries must be quantified correctly. Therefore, an accurate, sensitive and stable method for DNA quantification is crucial. In this study, seven different methods for DNA quantification were compared to each other by quantifying NGSlibraries for the Ion TorrentTM and Illumina1 platforms as well as dsDNA oligos with known DNA concentrations. Rather large variations in library concentration estimates were observed. The differences between the highest and lowest concentration estimates varied with a factor of 5–100 depending on thelibrary concentration. The Bioanalyzer, TapeStation and Qubit1 instruments gave concentrations closest to the expected when quantifying dsDNA oligos. At very low concentrations (2–4 pg/ul) only the Bioanalyzer could reliably quantify the dsDNA oligos.
AB - To ensure efficient sequencing, the DNA of next-generation sequencing (NGS) libraries must be quantified correctly. Therefore, an accurate, sensitive and stable method for DNA quantification is crucial. In this study, seven different methods for DNA quantification were compared to each other by quantifying NGSlibraries for the Ion TorrentTM and Illumina1 platforms as well as dsDNA oligos with known DNA concentrations. Rather large variations in library concentration estimates were observed. The differences between the highest and lowest concentration estimates varied with a factor of 5–100 depending on thelibrary concentration. The Bioanalyzer, TapeStation and Qubit1 instruments gave concentrations closest to the expected when quantifying dsDNA oligos. At very low concentrations (2–4 pg/ul) only the Bioanalyzer could reliably quantify the dsDNA oligos.
KW - Faculty of Health and Medical Sciences
KW - DNA quantification
KW - Next Generation Sequencing
KW - NanoDrop
KW - Qubit
KW - Fragment analyzer
KW - GX Touch
KW - Bioanalyzer
KW - TapeStation
KW - DNA quantification
KW - NanoDrop
KW - Qubit
KW - Next generation sequencing
KW - Fragment analyzer
KW - GX Touch
KW - Bioanalyzer
KW - TapeStation
U2 - 10.1016/j.fsigss.2015.09.110
DO - 10.1016/j.fsigss.2015.09.110
M3 - Conference article
VL - 5
SP - e276-e278
JO - Forensic Science International: Genetics. Supplement Series
JF - Forensic Science International: Genetics. Supplement Series
SN - 1875-1768
Y2 - 31 August 2015 through 5 September 2015
ER -
ID: 152269631