Comparing biological and pathological factors affecting osteocalcin concentrations in archaeological skeletal remains
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Comparing biological and pathological factors affecting osteocalcin concentrations in archaeological skeletal remains. / Scott, Amy B.; Taurozzi, Alberto; Hughes, Nicole; Pedersen, Dorthe Dangvard; Kontopoulos, Ioannis; Mackie, Meaghan; Collins, Matthew J.
I: Journal of Archaeological Science: Reports, Bind 34, Nr. Part A, 102573, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Comparing biological and pathological factors affecting osteocalcin concentrations in archaeological skeletal remains
AU - Scott, Amy B.
AU - Taurozzi, Alberto
AU - Hughes, Nicole
AU - Pedersen, Dorthe Dangvard
AU - Kontopoulos, Ioannis
AU - Mackie, Meaghan
AU - Collins, Matthew J.
PY - 2020
Y1 - 2020
N2 - Paleoproteomics and the study of ancient proteins has become an important consideration in bioarchaeological research as we seek to understand the relationship between the physical skeleton and its underlying biochemistry. Osteocalcin is an abundant, non-collagenous protein that is accessible archaeologically due to its affinity for hydroxyapatite in bone. Manufactured by the osteoblast cells during normal bone metabolism, osteocalcin is inherently linked to the process of bone remodelling. Clinical studies have consistently shown the impact of biological (i.e., sex and age) and pathological (i.e., disease) factors on osteocalcin concentrations within the body; however, this connection has yet to be fully explored bioarchaeologically. For this study, 46 individuals from the Danish Sct. Albert's cemetery (CE 1250–1536) were analyzed with cortical bone samples harvested from the femur. Each sample was demineralized and quantified using established paleoproteomic methods (ELISA) and tested for diagenesis using FTIR-ATR. No significant differences were found in the osteocalcin concentrations between adult males and females. However, males had a wider range of osteocalcin variability perhaps explained in part by their increased prevalence of pathological conditions characterized by bone remodelling. Similarly, non-adults had a wide range of osteocalcin variability but there were no significant differences when compared to the adult mean. No correlations were found between osteocalcin concentrations and diagenesis suggesting that the protein can survive across a range of temporal periods and variable burial environments. While no clear pattern emerged between osteocalcin concentrations and biological and/or pathological factors, this study is significant in that it further explores the use of paleoproteomics in bioarchaeological contexts and how we may unite our macroscopic and biochemical assessments of human skeletal remains.
AB - Paleoproteomics and the study of ancient proteins has become an important consideration in bioarchaeological research as we seek to understand the relationship between the physical skeleton and its underlying biochemistry. Osteocalcin is an abundant, non-collagenous protein that is accessible archaeologically due to its affinity for hydroxyapatite in bone. Manufactured by the osteoblast cells during normal bone metabolism, osteocalcin is inherently linked to the process of bone remodelling. Clinical studies have consistently shown the impact of biological (i.e., sex and age) and pathological (i.e., disease) factors on osteocalcin concentrations within the body; however, this connection has yet to be fully explored bioarchaeologically. For this study, 46 individuals from the Danish Sct. Albert's cemetery (CE 1250–1536) were analyzed with cortical bone samples harvested from the femur. Each sample was demineralized and quantified using established paleoproteomic methods (ELISA) and tested for diagenesis using FTIR-ATR. No significant differences were found in the osteocalcin concentrations between adult males and females. However, males had a wider range of osteocalcin variability perhaps explained in part by their increased prevalence of pathological conditions characterized by bone remodelling. Similarly, non-adults had a wide range of osteocalcin variability but there were no significant differences when compared to the adult mean. No correlations were found between osteocalcin concentrations and diagenesis suggesting that the protein can survive across a range of temporal periods and variable burial environments. While no clear pattern emerged between osteocalcin concentrations and biological and/or pathological factors, this study is significant in that it further explores the use of paleoproteomics in bioarchaeological contexts and how we may unite our macroscopic and biochemical assessments of human skeletal remains.
KW - Bioarchaeology
KW - Diagenesis
KW - ELISA
KW - FTIR-ATR
KW - Paleopathology
KW - Protein
U2 - 10.1016/j.jasrep.2020.102573
DO - 10.1016/j.jasrep.2020.102573
M3 - Journal article
AN - SCOPUS:85092315104
VL - 34
JO - Journal of Archaeological Science: Reports
JF - Journal of Archaeological Science: Reports
SN - 2352-409X
IS - Part A
M1 - 102573
ER -
ID: 250115319