Cold tolerance and freeze-induced glucose accumulation in three terrestrial slugs
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Cold tolerance and freeze-induced glucose accumulation in three terrestrial slugs. / Slotsbo, Stine; Hansen, Lars Monrad; Jordaens, Kurt; Backeljau, Thierry; Malmendal, Anders; Nielsen, Niels Chr; Holmstrup, Martin.
I: Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, Bind 161, Nr. 4, 2012, s. 443-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Cold tolerance and freeze-induced glucose accumulation in three terrestrial slugs
AU - Slotsbo, Stine
AU - Hansen, Lars Monrad
AU - Jordaens, Kurt
AU - Backeljau, Thierry
AU - Malmendal, Anders
AU - Nielsen, Niels Chr
AU - Holmstrup, Martin
N1 - Copyright © 2012 Elsevier Inc. All rights reserved.
PY - 2012
Y1 - 2012
N2 - Cold tolerance and metabolic responses to freezing of three slug species common in Scandinavia (Arion ater, Arion rufus and Arion lusitanicus) are reported. Autumn collected slugs were cold acclimated in the laboratory and subjected to freezing conditions simulating likely winter temperatures in their habitat. Slugs spontaneously froze at about -4 °C when cooled under dry conditions, but freezing of body fluids was readily induced at -1 °C when in contact with external ice crystals. All three species survived freezing for 2 days at -1 °C, and some A. rufus and A. lusitanicus also survived freezing at -2 °C. (1)H NMR spectroscopy revealed that freezing of body fluids resulted in accumulation of lactate, succinate and glucose. Accumulation of lactate and succinate indicates that ATP production occurred via fermentative pathways, which is likely a result of oxygen depletion in frozen tissues. Glucose increased from about 6 to 22 µg/mg dry tissue upon freezing in A. rufus, but less so in A. ater and A. lusitanicus. Glucose may thus act as a cryoprotectant in these slugs, although the concentrations are not as high as reported for other freeze tolerant invertebrates.
AB - Cold tolerance and metabolic responses to freezing of three slug species common in Scandinavia (Arion ater, Arion rufus and Arion lusitanicus) are reported. Autumn collected slugs were cold acclimated in the laboratory and subjected to freezing conditions simulating likely winter temperatures in their habitat. Slugs spontaneously froze at about -4 °C when cooled under dry conditions, but freezing of body fluids was readily induced at -1 °C when in contact with external ice crystals. All three species survived freezing for 2 days at -1 °C, and some A. rufus and A. lusitanicus also survived freezing at -2 °C. (1)H NMR spectroscopy revealed that freezing of body fluids resulted in accumulation of lactate, succinate and glucose. Accumulation of lactate and succinate indicates that ATP production occurred via fermentative pathways, which is likely a result of oxygen depletion in frozen tissues. Glucose increased from about 6 to 22 µg/mg dry tissue upon freezing in A. rufus, but less so in A. ater and A. lusitanicus. Glucose may thus act as a cryoprotectant in these slugs, although the concentrations are not as high as reported for other freeze tolerant invertebrates.
U2 - 10.1016/j.cbpa.2012.01.002
DO - 10.1016/j.cbpa.2012.01.002
M3 - Journal article
C2 - 22248916
VL - 161
SP - 443
EP - 449
JO - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
SN - 1095-6433
IS - 4
ER -
ID: 38224707