Chromatographic separation of alkaline phosphatase from dental enamel
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Chromatographic separation of alkaline phosphatase from dental enamel. / Moe, D; Kirkeby, S; Salling, E.
I: Scandinavian Journal of Dental Research, Bind 97, Nr. 1, 1989, s. 8-13.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Chromatographic separation of alkaline phosphatase from dental enamel
AU - Moe, D
AU - Kirkeby, S
AU - Salling, E
N1 - Keywords: Alkaline Phosphatase; Animals; Cattle; Chromatography; Chromatography, Gel; Chromatography, High Pressure Liquid; Dental Enamel; Dental Enamel Proteins; Electrophoresis; Isoelectric Focusing; Spectrophotometry
PY - 1989
Y1 - 1989
N2 - Alkaline phosphatase (AP) was prepared from partly mineralized bovine enamel by extraction in phosphate buffer, centrifugation and various chromatographic techniques. Chromatofocusing showed that the enamel enzyme possessed five isoelectric points at the acid pH level ranging from pH 5.7 to pH 4.4. Three enzyme peaks were eluted using low pressure chromatography with a Bio-gel column. With a HPLC gel filtration column the separation of the enamel extract resulted in only one peak with AP activity. The fractions of this peak were used to produce an antibody against bovine AP.
AB - Alkaline phosphatase (AP) was prepared from partly mineralized bovine enamel by extraction in phosphate buffer, centrifugation and various chromatographic techniques. Chromatofocusing showed that the enamel enzyme possessed five isoelectric points at the acid pH level ranging from pH 5.7 to pH 4.4. Three enzyme peaks were eluted using low pressure chromatography with a Bio-gel column. With a HPLC gel filtration column the separation of the enamel extract resulted in only one peak with AP activity. The fractions of this peak were used to produce an antibody against bovine AP.
M3 - Journal article
C2 - 2711125
VL - 97
SP - 8
EP - 13
JO - Scandinavian Journal of Dental Research
JF - Scandinavian Journal of Dental Research
SN - 0029-845X
IS - 1
ER -
ID: 10141289